Active Recombinant Mouse Il33 Protein

• Cat.No.: Il33-158M
• Product Overview: Purified recombinant protein of Mouse interleukin 33 (Il33), transcript variant 2 without tag was expressed in E.coli.

Specification

• Description: In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
• Source: E. coli
• Species: Mouse
• Bio-activity: ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is less than or equal to 0.5 ng/ml, corresponding to a specific activity of > 2 x 10^6units/mg.
• Molecular Mass: 17.5 kDa
• AA Sequence: SIQGTSLLTQSPASLSTYNDQSVSF VLENGCYVINVDDSGKDQEQDQVLL RYYESPCPASQSGDGVDGKKLMVNM SPIKDTDIWLHANDKDYSVELQRGD VSPPEQAFFVLHKKSSDFVSFECKN LPGTYIGVKDNQLALVEEKDESCNN IMFKLSKI
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Il33 interleukin 33 [ Mus musculus (house mouse) ]
• Official Symbol: Il33
• Synonyms: Il33; interleukin 33; Il-33; Il1f11; NF-HEV; 9230117N10Rik; interleukin-33; nuclear factor from high endothelial venules
• Gene ID: 77125
• mRNA Refseq: NM_133775
• Protein Refseq: NP_598536
• UniProt ID: Q8BVZ5