Recombinant Rat CD59 cell lysate

Cat.No. : CD59-1365RCL
Product Overview : Rat CD59 / CD59A / MAC / IP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cd59 CD59 molecule, complement regulatory protein [ Rattus norvegicus ]
Official Symbol : CD59
Synonyms : CD59; CD59 molecule, complement regulatory protein; CD59 glycoprotein; protectin; CD59 antigen; Cb59b molecule; MAC-inhibitory protein; membrane attack complex inhibition factor; CD59b moleucle, complement regulatory protein; Cd59a; Cd59b; MACIF; MACIP; MAC-IP;
Gene ID : 25407
mRNA Refseq : NM_012925
Protein Refseq : NP_037057
Pathway : Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem;
Function : complement binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How to explain the role of CD59 in hemolytic disease? 11/30/2022

In hemolytic diseases, the deficiency or dysfunction of CD59 may lead to excessive lysis of red blood cells because the formation of the complement C5b-9 complex cannot be effectively prevented.

Are there differences in CD59 expression levels in different types of cells? 09/30/2022

Yes, the expression level of CD59 can vary in different cell types. In general, it is expressed higher in blood cells and some tumor cells.

What are some of the technical challenges that laboratories may encounter when researching CD59? 12/03/2021

When researching CD59, laboratories may encounter technical challenges such as selecting the appropriate assay, ensuring the quality and consistency of samples, and the complexity of interpreting results. In addition, CD59 expression variations in different cell types and experimental conditions need to be considered.

What diseases are associated with aberrant expression of CD59? 07/19/2020

Aberrant expression of CD59 has been associated with a variety of diseases, including but not limited to hemolytic diseases, certain types of cancer, and autoimmune diseases.

How can I measure CD59 expression in the laboratory? 06/17/2020

CD59 expression can be detected using flow cytometry, immunofluorescence staining, or western blotting.

Is there a current treatment for CD59? 01/01/1900

The current treatment for CD59 is still in the research stage, and there is no drug approved for clinical use. However, some studies are exploring the use of gene therapy or antibody therapy to modulate CD59 expression or function.

Customer Reviews (3)

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Reviews
05/09/2022

    The quality is very high and the reliability is very helpful for our research results.

    03/17/2021

      There are no doping impurities, the experimental effect is very good, and I will recommend it to other students.

      01/13/2021

        Easy to operate and cost-effective.

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