Recombinant Rat IL23R cell lysate

Cat.No. : IL23R-1234RCL
Product Overview : Rat IL23R / IL23 Receptor derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il23r interleukin 23 receptor [ Rattus norvegicus ]
Official Symbol : IL23R
Synonyms : IL23R; interleukin 23 receptor;
Gene ID : 689932
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : contributes_to interleukin-12 receptor binding; contributes_to interleukin-23 binding; contributes_to interleukin-23 receptor activity; molecular_function;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Customer Reviews (3)

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    High specificity in ELISA.


      Effective in autoimmune models.


        Suitable for receptor binding studies.

        Q&As (10)

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        How does IL23R activation affect T-cell differentiation? 12/22/2022

        IL23R activation promotes Th17 cell differentiation.

        Does IL23R have alternative splicing variants? 02/20/2022

        IL23R has multiple alternative splicing variants.

        Does IL23R have any post-translational modifications that affect its stability? 08/23/2021

        IL23R undergoes phosphorylation that affects its stability.

        What is the half-life of IL23R protein in activated T-cells? 08/21/2020

        The half-life of IL23R protein in activated T-cells is approximately 48 hours.

        Are there any microRNAs that regulate IL23R expression? 06/16/2020

        MicroRNAs like miR-20a regulate IL23R expression.

        How does IL23R contribute to cytokine release? 02/15/2019

        IL23R contributes to the release of pro-inflammatory cytokines like IL-17.

        What role does IL23R play in autoimmune diseases? 08/22/2018

        IL23R is implicated in autoimmune diseases like Crohn's.

        How does IL23R expression change during infection? 10/12/2017

        IL23R expression is upregulated during bacterial infection.

        Does IL23R form homodimers or heterodimers? 08/15/2016

        IL23R forms heterodimers with IL-12Rβ1.

        What intracellular pathways are activated by IL23R? 08/06/2016

        IL23R activates the JAK-STAT and NF-κB pathways.

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