Active Recombinant Canine IL23R protein, His-tagged, Low Endotoxin

• Cat.No.: IL23R-1581C
• Product Overview: Recombinant Canine IL23R protein(A0A8I3PXL0)(24-354 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 24-354 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Canine IL-23R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 2.7μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.31 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.