Recombinant Human IL23R cell lysate
|Product Overview :||Human IL23R / IL23 Receptor derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
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|Source :||Human cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
|Gene Name :||IL23R interleukin 23 receptor [ Homo sapiens ]|
|Official Symbol :||IL23R|
|Synonyms :||IL23R; interleukin 23 receptor; interleukin-23 receptor; IL 23R; IL-23 receptor;|
|Gene ID :||149233|
|mRNA Refseq :||NM_144701|
|Protein Refseq :||NP_653302|
|UniProt ID :||Q5VWK5|
|Chromosome Location :||1p31.2|
|Pathway :||Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL23-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;|
|Function :||contributes_to interleukin-12 receptor binding; contributes_to interleukin-23 binding; contributes_to interleukin-23 receptor activity; receptor activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
IL23R activation promotes Th17 cell differentiation.
IL23R has multiple alternative splicing variants.
IL23R undergoes phosphorylation that affects its stability.
The half-life of IL23R protein in activated T-cells is approximately 48 hours.
MicroRNAs like miR-20a regulate IL23R expression.
IL23R contributes to the release of pro-inflammatory cytokines like IL-17.
IL23R is implicated in autoimmune diseases like Crohn's.
IL23R expression is upregulated during bacterial infection.
IL23R forms heterodimers with IL-12Rβ1.
IL23R activates the JAK-STAT and NF-κB pathways.
Customer Reviews (3)Write a review
High specificity in ELISA. -
Effective in autoimmune models. -
Suitable for receptor binding studies. -
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