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SMAD3

  • Official Full Name

    SMAD family member 3

  • Overview

    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
  • Synonyms

    SMAD3; SMAD family member 3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3;

  • Recombinant Proteins
  • Protein Pre-coupled Magnetic Beads
  • Cell & Tissue Lysates
  • Chicken
  • Human
  • Human/Mouse/Rat
  • Mouse
  • Rat
  • Rhesus Macaque
  • E.coli
  • HEK293
  • HEK293T
  • Human
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • His|MBP
  • SUMO
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human SMAD3-1094H Recombinant Human SMAD Family Member 3, GST-tagged E.coli GST
Human SMAD3-4599H Recombinant SMAD Family Member 3, His-tagged Human His
Human SMAD3-327H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP 241-425 aa
Human SMAD3-6915H Recombinant Human SMAD Family Member 3, His-tagged E.coli His 1-425aa
Human SMAD3-31191TH Recombinant Human SMAD3 E.coli N/A
Human SMAD3-31190TH Recombinant Human SMAD3, His-tagged E.coli His
Human SMAD3-295H Recombinant Human SMAD3 protein, GST-tagged E.coli GST
Human SMAD3-326H Recombinant Human SMAD3 protein, His/MBP-tagged E.coli His/MBP 16-221 aa
Human SMAD3-4788H Recombinant Human SMAD3 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human SMAD3-304H Recombinant Human SMAD3 Protein, His-tagged E.coli His Phe231~Ser425
Human SMAD3-1918H Recombinant Human SMAD3 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human SMAD3-227HFL Active Recombinant Full Length Human SMAD3 Protein, C-Flag-tagged Mammalian cells Flag
Human SMAD3-2256H Recombinant Human SMAD3 protein, His-tagged Insect Cell His 1-425aa
Human SMAD3-2041H-B Recombinant Human SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Human SMAD3-3506H Recombinant Human SMAD3 protein, His-SUMO-tagged E.coli His-SUMO 1-425aa
Human SMAD3-5867H Recombinant Human SMAD3 Protein (Asp220-Ser425), N-His tagged E.coli N-His Asp220-Ser425
Human SMAD3-1973HF Active Recombinant Full Length Human SMAD3 Protein, GST-tagged E.coli GST 1-425(end)
Human SMAD3-2041H Recombinant Human SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse SMAD3-532M Recombinant Mouse Smad3, His-GST tagged Insect Cell GST
Mouse SMAD3-001MCL Recombinant Mouse SMAD3 cell lysate Insect Cell N/A
Mouse Smad3-5951M Recombinant Mouse Smad3 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Smad3-305M Recombinant Mouse Smad3 Protein, His-tagged E.coli His Phe231~Ser425
Rat SMAD3-5608R Recombinant Rat SMAD3 Protein Mammalian Cell His
Rat SMAD3-5267R Recombinant Rat SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat SMAD3-5267R-B Recombinant Rat SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Human/Mouse/Rat Smad3-0256H Recombinant Human/Mouse/Rat Smad3 protein, His&GST-tagged Insect Cells His/GST Met1-Ser425
Rhesus Macaque SMAD3-4331R Recombinant Rhesus monkey SMAD3 Protein, His-tagged Mammalian Cell His
Rhesus Macaque SMAD3-4147R Recombinant Rhesus Macaque SMAD3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque SMAD3-4147R-B Recombinant Rhesus Macaque SMAD3 Protein Pre-coupled Magnetic Beads HEK293
Chicken SMAD3-6045C Recombinant Chicken SMAD3 Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • SMAD3 Related Articles
  • SMAD3 Related Gene Family
  • SMAD3 Related Research Area
  • SMAD3 Related Signal Pathway

SMAD3 involved in several pathways and played different roles in them. We selected most pathways SMAD3 participated on our site, such as FoxO signaling pathway, Cell cycle, Endocytosis, which may be useful for your reference. Also, other proteins which involved in the same pathway with SMAD3 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
FoxO signaling pathwaySMAD4;MAPK12B;PRKAG2;PIK3R5;PIK3CD;PIK3R1;MAPK11;PTEN;GADD45G
Cell cycleFZR1A;RAD1;MIS18BP1;RANGAP1B;TBC1D8;CDK5RAP2;CENPH;NCAPG2;TEX12
EndocytosisVPS45;AGAP2;ACAP1;TGFBR1A;HSPA1L;ARRB2A;RLN1;MET;ARPC1B
Wnt signaling pathwayNFATC3;PORCNL;PRKCA;LRP6;MAPK8;FZD8;FZD3A;FZD7A;MAPK8A
TGF-beta signaling pathwayTGFBR1A;PPP2CB;BMPR1AB;PITX2;MAPK3;BAMBI;BMP7B;TFDP1A;GDF6
Hippo signaling pathwayBIRC5;PRKCZ;GDF6;WNT2B;WNT4;WNT16;AREG;YAP1;LLGL2
Adherens junctionVCL;ACTN2B;RHOAC;ACTN3A;BAIAP2A;WAS;SMAD3;WASF1;CDH1
Signaling pathways regulating pluripotency of stem cellsINHBB;FZD8;FZD9;FGF2;GRB2;SMAD4;PIK3CB;ESX1;ESRRB
Chagas disease (American trypanosomiasis)PLCB4;GNA14;MAPK10;PPP2R2B;CXCL8;PIK3R5;PIK3CG;PPP2R1B;GNAO1
Hepatitis BYWHAZ;TLR3;EGR3;IFNA1;CCNE2;TICAM2;TIRAP;IKBKG;CREB3L1
HTLV-I infectionTRP53;MSX1;SMAD4;FDPS;AKT1;SLC25A4;HLA-G;CDC26;PTTG1
Pathways in cancerWNT9A;Fzd4;FGF22;ARNT;PIK3R3;MSH2;FLT3LG;PIK3CA;IL6
Colorectal cancerTGFB2;TGFB1;TP53;AKT1;BIRC5;APPL1;PIK3CG;MAPK1;Casp3
Pancreatic cancerRAC3;PIK3CG;IKBKG;STAT1;RAD51;BAD;TGFBR1;PIK3R3;CDC42
Chronic myeloid leukemiaABL1;PIK3R1;IKBKG;SOS1;PIK3R2;SMAD3;NFKBIA;CCND1;PIK3CB
Inflammatory bowel disease (IBD)IL4R;IL1A;TGFB2;RELA;Il23a;STAT3;IL12RB1;STAT1;GATA3

SMAD3 has several biochemical functions, for example, R-SMAD binding, RNA polymerase II activating transcription factor binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SMAD3 itself. We selected most functions SMAD3 had, and list some proteins which have the same functions with SMAD3. You can find most of the proteins on our site.

Function Related Protein
R-SMAD bindingPAX6;RANBP3;FOS;PMEPA1;PPM1A;MYOCD;ZEB2;SMAD4;TRIM33
RNA polymerase II activating transcription factor bindingPITX2;NFE2L2;TP53BP1;NCOR1;NHLH2;EGR2;LDB1;CTNNB1;SMAD3
RNA polymerase II core promoter proximal region sequence-specific DNA bindingSMAD2;FIGLA;JUNB;ZNF680;HHEX;ATOH1;ATF4;ZNF502;BCL11A
bHLH transcription factor bindingUSF2;TCF3;RUNX2;MAP3K10;IKZF4;ISL1;EP300;TCF12;USF1
beta-catenin bindingAr;SOX17;CHD8;TCF7L1A;LZIC;PROP1;TCF7L2;TRPC4;BCL9
chromatin DNA bindingSRF;SLAMF7;MYOD1;VAX2;FOXO3;WBP2;NKAP;VAX1;H2AFY
co-SMAD bindingTRIM33;CITED1;SMAD1;TGIF1;SMAD6;FOXH1;SMAD2;SMAD3;USP9X
collagen bindingCTSB;USH2A;Itga10&Itgb1;PDGFB;SMAD7;SERPINH1A;GP5;LACRT;SRGN
core promoter proximal region sequence-specific DNA bindingDMRTA1;CXXC1;DMRTC1B;SP3;GATA3;MEF2C;SMAD4;PAX2;KLF4
enhancer bindingELL3;FOXH1;TCF12;RAI1;MESP1;LEF1;SOX9;SP1;TCF3
identical protein bindingC1QTNF6;DAPK3;PACSIN2;OMG;PRKD1;P2RX2;PLEKHH2;TRA@;DCTPP1
phosphatase bindingSMAD2;TRPC4AP;CRY2;PPP1R26;TPRN;DLG3;PPP1R18;SH3RF2;PPP1R27
protein bindingWDR83;ALKBH4;CSDC2;IRF4;WASL;G6PD;CBS;GORASP2;RALGAPA1
protein heterodimerization activityPIK3R1;TAL1;ADRa1A;NR4A2;TIRAP;GABPA;AGTR1;OSTB;TAF6L
protein homodimerization activitySTOM;GPD1;DMRTB1;RAB11FIP2;DGAT2;IGJ;ABCD2;BCL2;MYOM3
protein kinase bindingPRKAG3;DUSP3;CCNL1B;DNM1;CDC25A;LATS1;PEBP1;SLC12A4;PARP1
sequence-specific DNA bindingSP1;SPDEF;DLX5A;RARGB;ZSCAN16;VSX2;ERF;FOXP1;HOXB13
transcription factor activity, RNA polymerase II core promoter sequence-specificHEY2;SMAD3;MAX;MEF2C;TFAP2B;PAX7;NKX3;HEYL;HEY1
contributes_to transcription factor activity, protein bindingSMAD3;SMAD4
transcription factor activity, sequence-specific DNA bindingZNF16;LHX1A;MYPOP;ATF4B2;HOXB4;TBX10;RAI1;PFDN1;NPAS1
contributes_to transcription factor activity, sequence-specific DNA bindingTRIM28;NKX2-5;HNRNPAB;FOXH1;SOX19A;SMAD2;NFE2L2A;NKX2;TAF10
transcription factor bindingBCL2;DNMT1;HMGB2;TFDP1A;AIP;PDX1;NKX2-5;KAT6B;MSX2
transcription regulatory region DNA bindingHNF4A;CDK5RAP2;TRP63;SALL4;ZNF217;MESP1;FOXI1;FOXM1;PAX8
transforming growth factor beta receptor bindingGDF1;USP15;GDF7;SMAD2;INHBE;LRG1;INHBA;GDF9;INHBB
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activitySMAD2;SMAD9;SMAD1;SMAD3
ubiquitin bindingNEDD4;UBXN1;VPS28;NBR1;SPRTN;CXCR4;KIF18A;UBXN11;FAM125A
ubiquitin protein ligase bindingPOU5F1;BAG4;LRPPRC;TRIB2;GPI1;PPARGC1A;NKD2;SPOP;HERC2
zinc ion bindingLMO7A;RXRB;PNMA3;RYBPA;NR2C2;ISL2;CHD4A;ZCCHC18;ZFP385C

SMAD3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SMAD3 here. Most of them are supplied by our site. Hope this information will be useful for your research of SMAD3.

SMAD4; SKI

Smithrithee, R; Niyonsaba, F; et al. Human beta-defensin-3 increases the expression of interleukin-37 through CCR6 in human keratinocytes. JOURNAL OF DERMATOLOGICAL SCIENCE 77:46-53(2015).
McMillin, M; Galindo, C; et al. Gli1 activation and protection against hepatic encephalopathy is suppressed by circulating transforming growth factor beta 1 in mice. JOURNAL OF HEPATOLOGY 61:1260-1266(2014).
TGF
  • Q&As
  • Reviews

Q&As (6)

Ask a question
What is the method used to determine the expression level and activity of SMAD3 protein? 12/05/2022

The expression level of SMAD3 protein can be detected by immunohistochemistry, western blotting, real-time PCR and other techniques. At the same time, the activity of SMAD3 protein can be assessed by detecting the phosphorylation level and the amount of SMAD3 protein within the nucleus.

How are the interactions between SMAD3 protein and other proteins regulated? 05/25/2022

interaction between SMAD3 protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, and interactions with other proteins. These factors can affect the interaction between SMAD3 protein and other proteins, and then affect the regulation process of TGF-β signal transduction pathway.

How is SMAD3 protein involved in the TGF-β signaling pathway? 04/28/2022

In the TGF-β signaling pathway, SMAD3 protein is activated by TGF-β to form complexes with other SMAD proteins, and then enters the nucleus to regulate the expression of specific genes, thereby affecting the growth, differentiation, apoptosis and immune response of cells.

How is the expression level of SMAD3 protein regulated? 03/05/2022

expression level of SMAD3 protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of SMAD3 protein, which in turn affects the growth, differentiation and apoptosis of cells.

What is the main function of SMAD3 protein? 01/25/2022

The main function of SMAD3 protein is to transmit signals in the TGF-β signal transduction pathway and regulate cell growth, differentiation, apoptosis and immune response.

What is SMAD3 protein? 07/22/2020

SMAD3 protein is a protein that plays a key role in intracellular signal transduction and belongs to the SMAD family. It is an effector molecule in the transforming growth factor-β (TGF-β) signaling pathway.

Customer Reviews (3)

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Reviews
05/14/2021

    High expression level, good stability, suitable for high precision experiments.

    03/08/2021

      Compared with other brands I have used, this brand is more stable and reliable.

      01/29/2021

        Strong antibacterial activity, not easy to be contaminated.

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