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SIGLEC6

  • Official Full Name

    sialic acid binding Ig-like lectin 6

  • Synonyms

    SIGLEC6; sialic acid binding Ig-like lectin 6; CD33L, CD33L1; sialic acid-binding Ig-like lectin 6; CD327; OB BP1; SIGLEC 6; CD33 antigen-like 1; obesity-binding protein 1; CD33L; OBBP1; CD33L1; CD33L2; CDW327;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Homo sapiens (Human)
  • Human
  • Rhesus monkey
  • CHO
  • E.coli
  • E.coli expression system
  • HEK293
  • Human Cell
  • Insect cell
  • Mammalian cells
  • C
  • His
  • Avi
  • Fc
  • hIgG4
  • Non
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human SIGLEC6-876H Active Recombinant Human SIGLEC6 Protein, Fc Chimera CHO Fc
Human SIGLEC6-146H Recombinant Human SIGLEC6, Fc tagged Human Cell His&Fc Gln27-Val331
Human SIGLEC6-59H Recombinant Human SIGLEC6, His-tagged HEK293 His Gln27-Val347
Human SIGLEC6-795HCL Recombinant Human SIGLEC6 cell lysate Human Cell Non
Human SIGLEC6-191H Recombinant Human SIGLEC6 Protein, His-tagged E.coli His
Human SIGLEC6-55H Recombinant Human SIGLEC6 Protein, Fc-His-tagged Insect cell His&Fc
Human SIGLEC6-559H Recombinant Human SIGLEC6 protein, His-Avi-tagged HEK293 C-His-Avi Gln27-Val331
Human SIGLEC6-339H Recombinant Human SIGLEC6 protein, Fc-tagged HEK293 Fc 305
Human SIGLEC6-1952H Recombinant Human SIGLEC6 protein, hFc-tagged HEK293 Fc Gln27-Val331
Human SIGLEC6-446H Recombinant Human SIGLEC6 Protein, Fc-tagged HEK293 Fc 453
Human SIGLEC6-13H Recombinant Human SIGLEC6 Protein (27-347), C-Fc-Avi tagged HEK293 Fc&Avi 27-347
Human SIGLEC6-5344H Recombinant Human SIGLEC6 Protein (Gln27-Ser331), C-Fc tagged Mammalian cells Fc Gln27-Ser331
Human SIGLEC6-0687H Recombinant Human SIGLEC6 protein, Fc-tagged HEK293 Fc Gln16-Val320
Human SIGLEC6-1086H Recombinant Human SIGLEC6 protein(Gln27-Val331), His-tagged HEK293 His Gln27-Val331
Rhesus monkey SIGLEC6-1743R Recombinant Rhesus Monkey SIGLEC6 Protein HEK293 Non 16-336
Rhesus monkey SIGLEC6-1744R Recombinant Rhesus Monkey SIGLEC6 Protein, hIgG1-tagged HEK293 Fc 16-336
Rhesus monkey SIGLEC6-1745R Recombinant Rhesus Monkey SIGLEC6 Protein, hIgG4-tagged HEK293 hIgG4 16-336
Homo sapiens (Human) RFL6654HF Recombinant Full Length Human Sialic Acid-Binding Ig-Like Lectin 6(Siglec6) Protein, His-Tagged E.coli expression system His Full Length of Mature Protein (27-453)
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is SIGLEC6 protein?

SIGLEC6 gene (sialic acid binding Ig like lectin 6) is a protein coding gene which situated on the long arm of chromosome 19 at locus 19q13. SIGLEC6, also known as CD327 or OB-BP1, is a cell surface receptor that belongs to the SIGLEC family. It is an immunomodulatory protein that is mainly expressed on immune cells such as macrophages, dendritic cells, natural killer cells, T cells, and B cells. SIGLEC6 has an immunoreceptor tyrosine motif inhibitory domain (ITIM) that regulates the immune response by interacting with tyrosine kinases within cells. SIGLEC6 functions include cell adhesion, intercellular recognition, and immune signaling. SIGLEC6 expression is also associated with placental function and is up-regulated in preeclampsia. The SIGLEC6 protein is consisted of 453 amino acids and SIGLEC6 molecular weight is approximately 49.9 kDa.

What is the function of SIGLEC6 protein?

SIGLEC6 is a putative adhesion molecule capable of mediating intercellular binding through sialic acid-dependent mechanisms. SIGLEC6 contains an immunoreceptor tyrosine motif inhibitory domain (ITIM) that regulates the immune response by interacting with tyrosine kinases within cells. Activation of SIGLEC6 can reduce inflammatory responses by inhibiting the activation of immune cells, for example, its expression on mast cells can regulate mast cell activation. SIGLEC6 mediates uptake of extracellular vesicles, possibly through atypical glycolipid binding pockets.

SIGLEC6-7.jpg

Fig1. Schema showing the mechanism of sTn-mediated Siglec-6 dependent migration of Siglec-6+ cells. (Jessica Nunes, 2024)

SIGLEC6 Related Signaling Pathway

SIGLEC6 can be used as a cell adhesion molecule to promote intercellular binding through sialic acid-dependent means, and participate in cell recognition and maintenance of tissue structure. SIGLEC6 contains the immunoreceptor tyrosine motif inhibitory domain (ITIM), which can regulate the activation and inhibition of immune cells through interaction with intracellular tyrosine kinases, affecting the immune response. SIGLEC6 may participate in the regulation of inflammatory response by regulating the activity of immune cells, and affect the pathological process of allergic diseases and inflammatory diseases. SIGLEC6 is expressed in certain types of cancer and may be involved in tumor aggressiveness, metastasis, and immune escape. For example, in chronic lymphocytic leukemia (CLL) and acute myeloid leukemia (AML), SIGLEC6 can be used as a target for CAR T cell therapy to target and inhibit tumor cell growth through specific CAR T cells.

SIGLEC6 Related Diseases

SIGLEC6 is an immunomodulatory protein whose abnormal expression is associated with a variety of diseases. In the field of cancer, SIGLEC6 expression is associated with chronic lymphocytic leukemia (CLL), acute myeloid leukemia (AML), and certain types of solid tumors (such as bladder cancer), and may promote tumor progression and metastasis by influencing immune cell activity in the tumor microenvironment. In addition, SIGLEC6 also plays a role in autoimmune diseases, such as in allergic diseases, SIGLEC6 acts as an inhibitory receptor on mast cells, and its activation may help regulate mast cell activation and degranulation, thereby affecting the occurrence of allergic diseases. SIGLEC6 also plays a role in pregnancy-related disorders, such as preeclampsia, whose expression is upregulated in the placenta and may be involved in the development of the disease.

Bioapplications of SIGLEC6

The regulatory role of SIGLEC6 makes it a target for drug development, and it may be beneficial for the treatment of related diseases by developing small molecule drugs or biologics that can regulate the activity of SIGLEC6. The expression level of SIGLEC6 may vary in different diseases and can be used as a biomarker for disease diagnosis, prognosis assessment and treatment response monitoring. In the field of cell therapy, such as CAR-T cell therapy, SIGLEC6 can be used as a specific marker for specific cell types to help improve the targeting and effectiveness of therapy. What's more, its manifestation in the disease makes it a potential therapeutic target.

High Purity

SDS-PAGE (SIGLEC6-191H).jpg

Fig1. SDS-PAGE (SIGLEC6-191H)

.

SDS-PAGE (SIGLEC6-339H).jpg

Fig2. SDS-PAGE (SIGLEC6-339H)

Case Study 1: Edward N Schmidt, 2023

Immunomodulatory Siglecs are controlled by their glycoprotein and glycolipid ligands. Siglec-glycolipid interactions are often studied outside the context of a lipid bilayer, missing the complex behaviors of glycolipids in a membrane. Through optimizing a liposomal formulation to dissect Siglec-glycolipid interactions, it is shown that Siglec-6 can recognize glycolipids independent of its canonical binding pocket, suggesting that Siglec-6 possesses a secondary binding pocket tailored for recognizing glycolipids in a bilayer. A panel of synthetic neoglycolipids is used to probe the specificity of this glycolipid binding pocket on Siglec-6, leading to the development of a neoglycolipid with higher avidity for Siglec-6 compared to natural glycolipids. This neoglycolipid facilitates the delivery of liposomes to Siglec-6 on human mast cells, memory B-cells and placental syncytiotrophoblasts. A physiological relevance for glycolipid recognition by Siglec-6 is revealed for the binding and internalization of extracellular vesicles.

SIGLEC6-1.jpg

Fig1. Binding of liposomes formulated with 3 mol% 3 and 4 to WT Siglec-6 CHO cells in the cell assay, relative to liposomes formulated without a ligand.

SIGLEC6-2.jpg

Fig2. Binding of 5 nGLLs to CHO cells expressing each Siglec-6/8 chimera.

Case Study 2: Kevin K W Lam, 2011

Glycodelin-A (GdA) is abundantly synthesized by the decidua but not the trophoblast. Previous data indicate that GdA suppresses the invasion of trophoblast cell lines by down-regulating proteinase expression and activities. This study addresses the signaling pathway involved in the above phenomenon. GdA was found to suppress phosphorylation of ERKs and expression of their downstream effector c-Jun, a component of the transcription factor activator protein-1 (AP-1). The involvement of ERKs and c-Jun in suppressing trophoblast invasion and biosynthesis of proteinases was confirmed by using siRNA knockdown and pharmacological inhibitors. Co-immunoprecipitation showed that Siglec-6 on the trophoblast was the binding protein of GdA. The binding of GdA to Siglec-6 was sialic acid-dependent. Treatment with anti-Siglec-5 antibody abolished the invasion suppressive activities of GdA.

SIGLEC6-3.jpg

Fig3. Western blot analysis of the interaction between recombinant Siglec-6 chimeric protein and either GdA or desialylated GdA.

SIGLEC6-4.jpg

Fig4. Effect of Siglec-6 antibody on JEG-3 cell invasion.

SIGLEC6 involved in several pathways and played different roles in them. We selected most pathways SIGLEC6 participated on our site, such as Adaptive Immune System, Immune System, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, which may be useful for your reference. Also, other proteins which involved in the same pathway with SIGLEC6 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Adaptive Immune SystemCTSO;TPP2;DNM1;KLHL20;CYBA;SPSB2;CD300A;ASB14;NRG3
Immune SystemASB18;WSB1;SEC24A;ATP6V1G3;DEFB106A;BST2;ACTR1B;LILRB5;CMTM2A
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cellMICA;SH2D1B;CD200;KLRB1B;CLEC2H;GM11711;RAET1E;CD300LD;TREM1

SIGLEC6 has several biochemical functions, for example, carbohydrate binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SIGLEC6 itself. We selected most functions SIGLEC6 had, and list some proteins which have the same functions with SIGLEC6. You can find most of the proteins on our site.

Function Related Protein
carbohydrate bindingITLN1;CLEC4D;GALNT9;LGALS2B;GALNT18A;Clec4n;KLRC2;CLEC14A;FCNA
protein bindingPHB2;TEN1;CASP2;MTIF3;STX1A;FER;RCAN3;TMPRSS4;HEBP2

SIGLEC6 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SIGLEC6 here. Most of them are supplied by our site. Hope this information will be useful for your research of SIGLEC6.

TCF4; FHL2; rfbD; y936_yerpe

Research Area

Related articles

Charlton, JR; Norwood, VF; et al. Evolution of the urinary proteome during human renal development and maturation: variations with gestational and postnatal age. PEDIATRIC RESEARCH 72:179-185(2012).
Kardava, L; Moir, S; et al. Attenuation of HIV-associated human B cell exhaustion by siRNA down regulation of inhibitory receptors. JOURNAL OF CLINICAL INVESTIGATION 121:2614-2624(2011).
  • Reviews
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Customer Reviews (3)

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Reviews
10/28/2022

    Their expertise and prompt assistance can effectively address any concerns or challenges that may arise during the experimental process.

    06/30/2022

      In addition to its outstanding quality, the manufacturer of the SIGLEC6 protein provides excellent technical support, which is a valuable asset for researchers

      10/24/2016

        The SIGLEC6 protein is of exceptional quality, making it an ideal choice to meet my experimental requirements.

        Q&As (5)

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        What are the future prospects for the clinical application of SIGLEC6 protein? 08/16/2021

        The clinical application of SIGLEC6 protein holds promise for advancing the diagnosis, treatment, and monitoring of autoimmune diseases, inflammatory conditions, and cancer, with ongoing research paving the way for potential therapeutic interventions.

        Are there any genetic variations in SIGLEC6 protein associated with disease susceptibility? 04/02/2021

        Genetic variations in the SIGLEC6 gene have been investigated for their potential association with disease susceptibility, particularly in autoimmune and inflammatory conditions.

        How does SIGLEC6 protein interact with other immune receptors? 08/29/2018

        SIGLEC6 protein has been shown to interact with other immune receptors, potentially influencing the overall immune response and its contribution to disease pathology.

        Can SIGLEC6 protein be targeted for drug development? 06/27/2017

        Given its involvement in immune regulation and inflammatory processes, SIGLEC6 protein is a potential target for drug development in the treatment of autoimmune and inflammatory diseases.

        Can SIGLEC6 protein be used as a therapeutic target in immunotherapy? 05/17/2016

        The potential use of SIGLEC6 protein as a therapeutic target in immunotherapy is an area of active investigation, particularly in the context of modulating immune responses in cancer treatment.

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