Recombinant Human SIGLEC6 protein, Fc-tagged SIGLEC6-339H

Recombinant Human SIGLEC6 protein, Fc-tagged

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Recombinant Human SIGLEC6 protein, Fc-tagged

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Cat.No. : SIGLEC6-339H
Product Overview : Recombinant Human SIGLEC6 protein (Gln16-Val320), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Sialic acid-binding Ig-like lectin 6 (SIGLEC6) is also known as CD antigen CD327, CD33 antigen-like 1 (CD33L or CD33L1), Obesity-binding protein 1 (OB-BP1). SIGLEC6 belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family, which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIGLEC6 mediates sialic-acid dependent binding to cells. SIGLEC6 binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 60.3 kDa. As a result of glycosylation, the protein migrates as 80-90 kDa under reducing (R) condition, and 160-180 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : 305
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of SIGLEC6-339H :

Human Siglec-6, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Gene Name : SIGLEC6
Official Symbol : SIGLEC6
Synonyms : SIGLEC6; sialic acid binding Ig-like lectin 6; CD33L, CD33L1; sialic acid-binding Ig-like lectin 6; CD327; OB BP1; SIGLEC 6; CD33 antigen-like 1; obesity-binding protein 1; CD33L; OBBP1; CD33L1; CD33L2; CDW327
Gene ID : 946
mRNA Refseq : NM_001177547
Protein Refseq : NP_001171018
MIM : 604405
UniProt ID : O43699

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