Active Recombinant Full Length Human NCL Protein, C-Flag-tagged

• Cat.No.: NCL-03HFL
• Product Overview: Recombinant Full Length Human NCL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA reaction positive control
  Taq polymerase assay (regulator)
  Binding assay (Dimethylsulfate footprinting)
  Binding assay (FRET)
  Surface Plasmon Ressonance (SPR)
  Association in cell culture
  Surface Plasmon Ressonance (SPR)
  EMSA reaction positive control
  ELISA binding assay
• Molecular Mass: 76.4 kDa
• AA Sequence: MVKLAKAGKNQGDPKKMAPPPKEVE EDSEDEEMSEDEEDDSSGEEVVIPQ KKGKKAAATSAKKVVVSPTK KVAVATPAKKAAVTPGKKAAATPAK KTVTPAKAVTTPGKKGATPGKALVA TPGKKGAAIPAKGAKNGKNA KKEDSDEEEDDDSEEDEEDDEDEDE DEDEIEPAAMKAAAAAPASEDEDDE DDEDDEDDDDDEEDDSEEEA METTPAKGKKAAKVVPVKAKNVAED EDEEEDDEDEDDDDDEDDEDDDDED DEEEEEEEEEEPVKEAPGKR KKEMAKQKAAPEAKKQKVEGTEPTT AFNLFVGNLNFNKSAPELKTGISDV FAKNDLAVVDVRIGMTRKFG YVDFESAEDLEKALELTGLKVFGNE IKLEKPKGKDSKKERDARTLLAKNL PYKVTQDELKEVFEDAAEIR LVSKDGKSKGIAYIEFKTEADAEKT FEEKQGTEIDGRSISLYYTGEKGQN QDYRGGKNSTWSGESKTLVL SNLSYSATEETLQEVFEKATFIKVP QNQNGKSKGYAFIEFASFEDAKEAL NSCNKREIEGRAIRLELQGP RGSPNARSQPSKTLFVKGLSEDTTE ETLKESFDGSVRARIVTDRETGSSK GFGFVDFNSEEDAKAAKEAM EDGEIDGNKVTLDWAKPKGEGGFGG RGGGRGGFGGRGGGRGGRGGFGGRG RGGFGGRGGFRGGRGGGGDH
  KPQGKKTKFETRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Pathogenic Escherichia coli infection

Gene Information

• Gene Name: NCL nucleolin [ Homo sapiens (human) ]
• Official Symbol: NCL
• Synonyms: C23; Nsr1
• Gene ID: 4691
• mRNA Refseq: NM_005381.3
• Protein Refseq: NP_005372.2
• MIM: 164035
• UniProt ID: P19338