MAP1LC3B-192H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human MAP1LC3B (amino acid residues 1-120), fused with N-terminal His, was expressed in E.coli.
Description :
Three classes of enzymes are involved in autophagy; E1-like activating enzymes (E1s), E2-like conjugating enzymes (E2s) and ubiquitin-like proteins (ubls). Microtubule Associated Protein 1, Light Chain 3 beta, MAP1LC3b or LC3b is a member of the Ubl family and cloning of the human gene was first described by He et al. (2003). The C-terminal of LC3b is cleaved by ATG4B C-terminal to Gly120 in vitro and this has been shown to be required for the formation of intermediates with the ubiquitin-like activating enzyme ATG7 (Tanida et al. 2004).
Source :
E. coli
Species :
Human
Tag :
His
Form :
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity :
E1 Thioester LC3b Loading Assay: The activity of LC3b was validated by loading LC3b onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of LC3b and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the LC3b/His-ATG7 thioester bond to the reducing agent DTT was confirmed.
Molecular Mass :
~16.93 kDa
Purity :
>98% by SDS-PAGE
Storage :
12 months at -70°C. Avoid multiple freeze/thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.