Active Recombinant Human α Chymotrypsin

• Cat.No.: α Chymotrypsin-19H
• Product Overview: Our recombinant human α-chymotrypsin is expressed in Pichia pastoris and obtained through multiple purification steps, without contamination from other proteases.

Specification

• Species: Human
• Source: Yeast
• Tag: Non
• Description: α-Chymotrypsin is a serine protease that can hydrolyze the carboxy-terminal peptide bonds of tyrosine, tryptophan, and phenylalanine. Ca2+ serves as an activator and stabilizer for α-chymotrypsin. Meanwhile, this enzyme is inhibited by DFP, PMSF, TPCK, chymotrypsin inhibitor, aprotinin, α1-antitrypsin and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
• Form: Lyophilized
• Purity: ≥ 90% by SDS-PAGE
• Bio-activity: ≥ 70 U/mg
• Protein Content: ≥ 20 μg
• Unit definition: One unit (U) is defined as the amount of enzyme that hydrolyzes 1.0 μmol of BTEE per minute under the conditions of pH 7.8 and 25 centigrade.
• Usage: 1. It is recommended to centrifuge this product at 100-200g for 2 minutes before use. Then, carefully open the lid and add 1 mM HCl for reconstitution to a final concentration of 1 mg/mL. After that, close the tube lid and invert the tube up-and-down or shake it to mix thoroughly. After reconstitution, this product can be stored at 4 centigrade or -20 centigrade for at least 3 months. Try to avoid repeated freeze-thaw cycles after reconstitution. 2. After reconstitution, this product can be directly applied to peptide mapping analysis or protein identification. The recommended addition ratio is 1: 20-1: 200, and the digestion is carried out at 25 centigrade for 2-18 h.
• Applications: 1. Peptide mapping analysis 2. Protein identification
• Note: 1. After reconstitution, try to avoid repeated freeze-thaw cycles for this product; 2. Please wear lab coat and disposable gloves when using; 3. This product should not be used directly for clinical diagnosis and treatment.
• Stability: This product should be stored at 2-8 centigrade and can be stored for at least 12 months.
• Storage: At 2-8 centigrade
• Shipping: Dry ice or ice pack
• Reconstitution: 1 mM HCl