Active Recombinant Mouse Gdf9

• Cat.No.: Gdf9-180M
• Product Overview: Recombinant Mouse Gdf9 (Accession # AAA53035) Gly307-Arg441 was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Predicted N Terminal: Gly307
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.100 ng/mL rmGDF-9 can effectively induce Smad2 phosphorylation.
• Molecular Mass: Recombinant Mouse Gdf9 has a calculated MW of 15.6 kDa. In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing 0.1% bovine serum albumin.
• Tag: Non
• Protein length: Gly307-Arg441

Gene Information

• Gene Name: Gdf9 growth differentiation factor 9 [ Mus musculus ]
• Official Symbol: Gdf9
• Synonyms: GDF9; growth differentiation factor 9; growth/differentiation factor 9; Gdf-9
• Gene ID: 14566
• mRNA Refseq: NM_008110
• Protein Refseq: NP_032136

Reviews

11/14/2021

Expertise in peptide synthesis, accelerates our peptide-related projects.

08/24/2020

Swift protein quantification, improves data reliability in our experiments.

01/31/2019

Outstanding protein-protein interaction studies, boost our research efforts.

Q&As

How does GDF9 influence the proliferation and differentiation of granulosa cells in the ovary?

GDF9 stimulates granulosa cell proliferation and differentiation, essential for healthy ovarian function.

What are the mechanisms by which GDF9 modulates intra-ovarian signaling pathways?

GDF9 interacts with intra-ovarian signaling pathways, coordinating follicle development and ovulation.

How do alterations in GDF9 expression or function affect embryonic development and early pregnancy?

Changes in GDF9 expression impact embryonic development and the success of early pregnancy stages.

What role does GDF9 play in fertility and reproductive outcomes in females?

GDF9 significantly impacts female fertility, affecting egg quality and reproductive potential.

What is the impact of GDF9 mutations on the pathogenesis of ovarian dysfunctions and disorders?

Mutations in GDF9 can lead to ovarian disorders, impacting follicle development and female fertility.

How does GDF9 regulate ovarian follicle development and oocyte maturation?

GDF9 is crucial for ovarian follicle growth and oocyte maturation, influencing female fertility.

How does GDF9 contribute to the regulation of steroidogenesis in ovarian cells?

GDF9 regulates steroid hormone production in ovarian cells, influencing reproductive hormone balance.