Recombinant Human GDF9 293 Cell Lysate

• Cat.No.: GDF9-5966HCL

Specification

• Description: Antigen standard for growth differentiation factor 9 (GDF9) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: GDF9 growth differentiation factor 9 [ Homo sapiens ]
• Official Symbol: GDF9
• Synonyms: GDF9; growth differentiation factor 9; growth/differentiation factor 9; GDF-9
• Gene ID: 2661
• mRNA Refseq: NM_005260
• Protein Refseq: NP_005251
• MIM: 601918
• UniProt ID: O60383
• Chromosome Location: 5q31.1
• Pathway: Ovarian Infertility Genes, organism-specific biosystem
• Function: cytokine activity; growth factor activity

Reviews

11/14/2021

Expertise in peptide synthesis, accelerates our peptide-related projects.

08/24/2020

Swift protein quantification, improves data reliability in our experiments.

01/31/2019

Outstanding protein-protein interaction studies, boost our research efforts.

Q&As

How does GDF9 influence the proliferation and differentiation of granulosa cells in the ovary?

GDF9 stimulates granulosa cell proliferation and differentiation, essential for healthy ovarian function.

What are the mechanisms by which GDF9 modulates intra-ovarian signaling pathways?

GDF9 interacts with intra-ovarian signaling pathways, coordinating follicle development and ovulation.

How do alterations in GDF9 expression or function affect embryonic development and early pregnancy?

Changes in GDF9 expression impact embryonic development and the success of early pregnancy stages.

What role does GDF9 play in fertility and reproductive outcomes in females?

GDF9 significantly impacts female fertility, affecting egg quality and reproductive potential.

What is the impact of GDF9 mutations on the pathogenesis of ovarian dysfunctions and disorders?

Mutations in GDF9 can lead to ovarian disorders, impacting follicle development and female fertility.

How does GDF9 regulate ovarian follicle development and oocyte maturation?

GDF9 is crucial for ovarian follicle growth and oocyte maturation, influencing female fertility.

How does GDF9 contribute to the regulation of steroidogenesis in ovarian cells?

GDF9 regulates steroid hormone production in ovarian cells, influencing reproductive hormone balance.