Active Recombinant Human GDF9

• Cat.No.: GDF9-999H
• Product Overview: Recombinant Human GDF9 (Accession # O60383) Gly320-Arg454, fused with , was produced in Human embryonic kidney cell, HEK293-derived.

Specification

• Source: HEK293 Cells
• Species: Human
• Predicted N Terminal: Gly320
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.50-100 ng/mL of Recombinant Human GDF-9 can effectively induce Smad2 phosphorylation.
• Molecular Mass: Recombinant Human GDF9 has a calculated MW of 16 kDa (monomer). In SDS-PAGE migrates as 20-22 kDa, reducing conditions.
• Protein length: Gly320-Arg454
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GDF9 growth differentiation factor 9 [ Homo sapiens ]
• Official Symbol: GDF9
• Synonyms: GDF9; growth differentiation factor 9; growth/differentiation factor 9; GDF-9
• Gene ID: 2661
• mRNA Refseq: NM_005260
• Protein Refseq: NP_005251
• MIM: 601918
• UniProt ID: O60383
• Chromosome Location: 5q31.1