Active Recombinant Mouse Il27, Fc-tagged

• Cat.No.: Il27-351M
• Product Overview: The mouse IL-27 complex composed of the Ebi3 subunit (aa 1-228) and the p28 subunit (aa 29-234) is fused through a polypeptide linker to the Fc region of human IgG1.

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Protein Length: 1-228;29-234 a.a.
• Description: Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an antiinflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on na?e CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction.
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.
• Purity: ≥98% (SDS-PAGE)
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Official Symbol: Il27
• Synonyms: IL27; interleukin 27; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; p28; Il30; IL-27; IL-27p28
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• Function: cytokine activity; interleukin-27 receptor binding; protein binding; receptor binding