Active Recombinant Human IL27 protein, Fc-tagged

• Cat.No.: IL27-150H
• Product Overview: Recombinant Human IL27 composed of IL27B subunit (NP_005746.2) (Phe 29 - Pro 243) and the N-terminally truncated IL27p28 subunit (NP_663634.2) (Arg 21 - Lys 229) linked by an polypeptide linker, fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 29-243 aa&21-229 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-27 Protein, Fc Tag, premium grade at 5 μg/mL (100 μL/well) can bind Human IL-27 Ra Protein, His Tag with a linear range of 0.008-0.25 μg/mL.
• Molecular Mass: The protein has a calculated MW of 75.9 kDa. The protein migrates as 44 kDa, 48 kDa and 90-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method / rFC method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL27 interleukin 27 [ Homo sapiens ]
• Official Symbol: IL27
• Synonyms: IL27; interleukin 27
• Gene ID: 144841