Recombinant Human IL27 cell lysate

• Cat.No.: IL27-001HCL
• Product Overview: Human IL27 / Interleukin-27 derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: CHO cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL27 interleukin 27 [ Homo sapiens ]
• Official Symbol: IL27
• Synonyms: IL27; interleukin 27
• Gene ID: 144841

Reviews

03/30/2022

The purity is very high, no impurities.

08/05/2020

Biological activity is very strong and can be used in various biological experiments.

05/30/2019

Stability is very good, and it will not denaturate at high temperatures.

Q&As

Are there have current difficulties in the study of Il27?

The current challenges in the study of Il27 include potential side effects, preparation purity and other challenges.

Which scientific research fields are deeply studying Il27?

The potential application of Il27 in tumor immunotherapy and autoimmune diseases is being deeply studied in the scientific research field.

Is the production of Il27 related to genetic factors?

The production of Il27 is related to genetic factors, and the expression level and polymorphism of genes may affect its production and function.

Which role of Il27 in inflammatory diseases?

Studies have shown that Il27 may have an anti-inflammatory effect in inflammatory diseases, regulating the degree of inflammatory response.

What the potential applications of recombinant Il27 in cancer therapy?

Current studies have shown that Il27 has potential application value in enhancing anti-tumor immunity and alleviating tumor invasion and metastasis.

What is the effect of Il27 on immune response?

Il27 can enhance the activation and proliferation of specific immune cells and regulate the intensity and direction of immune response.