Agarose 2B
Cat.No. : | Agarose-001A |
Product Overview : | Agarose 2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. |
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Cat.no : | Agarose-001A |
Matrix : | Agarose, 2% |
Average particle size : | 60 μm-200 μm |
Chemical stability : | Stable to all solutions commonly used in gel filtration including 8 M Urea and 6 M guanidine hydrochloride |
Physical stability : | Negligible volume variation due to changes in pH or ionic strength |
pH working range : | 4-9 |
pH CIP range : | 4-9 |
Tag : | Non |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (5)
Write a reviewReplicating outcomes across diverse cell lines underscored the product’s consistent behavior.
Our data indicates that Agarose promotes neurite outgrowth effectively.
The product’s insights justified its cost, representing a valuable investment in our research.
It’s reliability was evident in consistent protein-protein interactions.
It's purity enhanced the accuracy of mass spectrometry results.
Q&As (21)
Ask a questionYes, agarose can vary in its gel strength and pore size, affecting its ability to separate molecules of different sizes.
Agarose gel electrophoresis is primarily used for nucleic acids (DNA, RNA) separation. Proteins are often separated using polyacrylamide gels (SDS-PAGE).
The buffer maintains a stable pH and provides ions to carry the electric current, facilitating the movement of molecules through the gel.
Agarose gel electrophoresis provides qualitative information about molecular size, but not precise quantification of amounts.
Agarose concentration determines the size range of molecules that can be separated; lower concentration gels are better for larger molecules, and vice versa.
No, agarose gels do not provide a suitable matrix for separating proteins based on charge; that's better achieved with techniques like isoelectric focusing.
No, agarose gels provide relatively low resolution compared to techniques like capillary electrophoresis or high-resolution polyacrylamide gels.
Loading dye helps visualize sample loading and migration during electrophoresis. It adds density to the samples, making them sink into the wells, and provides color for tracking progress.
Yes, agarose gels can be used for isolating DNA fragments of interest by cutting out the desired band from the gel.
The comb creates wells in the gel where samples are loaded. After the gel solidifies, the comb is removed, leaving empty wells for sample loading.
Agarose gel electrophoresis involves placing DNA, RNA, or proteins in wells on an agarose gel, applying an electric field, and watching them migrate through the gel matrix based on their size.
Agarose gels are not typically used for separating protein complexes; techniques like native PAGE or blue native PAGE are better suited for this purpose.
Protein samples are often mixed with a loading buffer that provides color and density for loading, and sometimes a reducing agent to break disulfide bonds.
Yes, agarose gel electrophoresis can also separate RNA molecules based on their size, similar to how it separates DNA.
A DNA ladder consists of DNA fragments of known sizes. It helps estimate the size of unknown DNA fragments by comparing their migration distances to those of the ladder's fragments.
While agarose gels are not ideal for protein separation, native protein conformation can be preserved, and small proteins may be separated to some extent.
Agarose gel provides a porous medium through which biomolecules can move, separating them by size as smaller molecules migrate faster through the gel.
Yes, polyacrylamide gels (SDS-PAGE) are commonly used for protein separation due to their higher resolution and ability to denature proteins.
Agarose is a polysaccharide derived from seaweed, commonly used in gel electrophoresis for separating DNA, RNA, and proteins.
Higher voltages can lead to faster migration but may cause heat buildup and distortion. Optimal voltage depends on the gel's thickness and size of molecules being separated.
DNA is often stained with fluorescent dyes, like ethidium bromide or SYBR Green, which bind to DNA and emit visible light under UV illumination.
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