AHCY Inhibitor Screening Kit
Cat.No. : | Kit-2151 |
Product Overview : | In the AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent. |
- Specification
- Gene Information
- Related Products
Size : | 100 assays |
Applications : | Screening/characterizing/studying AHCY inhibitors |
Storage : | Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay.AHCY Assay Buffer: Store at 4°C or -20°C. Warm to 37°C before use.AHCY Enzyme: Reconstitute with 220 µl AHCY Reconstitution Buffer, keep on ice, and mix gently by pipetting. Aliquot and store at -80°C. Avoid repeated freeze/thaw. After opening, use within two months.Adenosine Deaminase: Reconstitute with 110 µl deionized water. Avoid repeated freeze/thaw. Aliquot and store at -80°C. After opening, use within two months.3-Deazaneplanocin A (10 µM): Store at -20°C. After opening, use within two months.AHCY Substrate: Store at -20°C. Thaw and mix gently by pipetting before use. After opening, use within two months.Thiol detecting Reagent: Store at -20°C. Thaw before use. After opening, use within two months. |
Products Types
◆ Recombinant Protein | ||
AHCY-403M | Recombinant Mouse AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
AHCY-519H | Recombinant Human AHCY Protein, MYC/DDK-tagged | +Inquiry |
AHCY-227R | Recombinant Rat AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
AHCY-294H | Recombinant Human AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
Ahcy-1564M | Recombinant Mouse Ahcy Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
AHCY-8964HCL | Recombinant Human AHCY 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2302 | Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionBy degrading SAH, AHCY ensures the availability of S-adenosylmethionine (SAM), a universal methyl donor essential for DNA, RNA, and protein methylation reactions.
Dysregulation of AHCY and altered methylation patterns have been linked to the development and progression of NAFLD and other liver pathologies.
AHCY employs a zinc-dependent mechanism to cleave the N-ribosidic bond of SAH, generating adenosine and homocysteine as products.
Targeting AHCY activity or SAM availability holds promise as a potential therapeutic strategy for conditions influenced by disrupted methylation.
Single nucleotide polymorphisms (SNPs) in the AHCY gene can affect enzyme activity and SAH levels, potentially influencing disease risk, including cardiovascular disorders and neurodegenerative diseases.
Homocysteine can be remethylated to methionine through the folate-dependent pathway or transsulfurated to cysteine via the transsulfuration pathway.
AHCY activity is intimately connected with folate-mediated one-carbon metabolism, affecting the availability of methyl groups for various methylation reactions.
AHCY expression can be modulated by transcriptional factors and epigenetic modifications, responding to changes in cellular methylation needs.
AHCY indirectly influences polyamine biosynthesis by providing the methyl groups required for polyamine synthesis.
AHCY possesses a TIM-barrel fold with an active site that houses the zinc ion critical for its hydrolytic activity.
Customer Reviews (5)
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This protein is like a reliable teammate in the lab – always delivering excellent results.
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