AHCY Inhibitor Screening Kit Kit-2151

AHCY Inhibitor Screening Kit

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AHCY Inhibitor Screening Kit

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Cat.No. : Kit-2151
Product Overview : In the AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
Size : 100 assays
Applications : Screening/characterizing/studying AHCY inhibitors
Storage : Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay.AHCY Assay Buffer: Store at 4°C or -20°C. Warm to 37°C before use.AHCY Enzyme: Reconstitute with 220 µl AHCY Reconstitution Buffer, keep on ice, and mix gently by pipetting. Aliquot and store at -80°C. Avoid repeated freeze/thaw. After opening, use within two months.Adenosine Deaminase: Reconstitute with 110 µl deionized water. Avoid repeated freeze/thaw. Aliquot and store at -80°C. After opening, use within two months.3-Deazaneplanocin A (10 µM): Store at -20°C. After opening, use within two months.AHCY Substrate: Store at -20°C. Thaw and mix gently by pipetting before use. After opening, use within two months.Thiol detecting Reagent: Store at -20°C. Thaw before use. After opening, use within two months.

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