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AHCY Inhibitor Screening Kit

Cat.No. : Kit-2151
Product Overview : In the AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
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Size : 100 assays
Applications : Screening/characterizing/studying AHCY inhibitors
Storage : Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay.AHCY Assay Buffer: Store at 4°C or -20°C. Warm to 37°C before use.AHCY Enzyme: Reconstitute with 220 µl AHCY Reconstitution Buffer, keep on ice, and mix gently by pipetting. Aliquot and store at -80°C. Avoid repeated freeze/thaw. After opening, use within two months.Adenosine Deaminase: Reconstitute with 110 µl deionized water. Avoid repeated freeze/thaw. Aliquot and store at -80°C. After opening, use within two months.3-Deazaneplanocin A (10 µM): Store at -20°C. After opening, use within two months.AHCY Substrate: Store at -20°C. Thaw and mix gently by pipetting before use. After opening, use within two months.Thiol detecting Reagent: Store at -20°C. Thaw before use. After opening, use within two months.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the significance of AHCY in maintaining cellular methylation homeostasis? 08/22/2022

By degrading SAH, AHCY ensures the availability of S-adenosylmethionine (SAM), a universal methyl donor essential for DNA, RNA, and protein methylation reactions.

Explain the involvement of AHCY in the pathogenesis of liver diseases, such as non-alcoholic fatty liver disease (NAFLD). 06/17/2022

Dysregulation of AHCY and altered methylation patterns have been linked to the development and progression of NAFLD and other liver pathologies.

How does AHCY catalyze the hydrolysis of S-adenosylhomocysteine? 04/02/2022

AHCY employs a zinc-dependent mechanism to cleave the N-ribosidic bond of SAH, generating adenosine and homocysteine as products.

Describe the AHCY-related therapeutic approaches for diseases linked to aberrant methylation patterns. 12/05/2021

Targeting AHCY activity or SAM availability holds promise as a potential therapeutic strategy for conditions influenced by disrupted methylation.

How do genetic variations in AHCY contribute to disease susceptibility? 02/09/2021

Single nucleotide polymorphisms (SNPs) in the AHCY gene can affect enzyme activity and SAH levels, potentially influencing disease risk, including cardiovascular disorders and neurodegenerative diseases.

What are the metabolic fates of homocysteine generated by AHCY activity? 12/14/2020

Homocysteine can be remethylated to methionine through the folate-dependent pathway or transsulfurated to cysteine via the transsulfuration pathway.

Discuss the crosstalk between AHCY and folate metabolism in one-carbon metabolism. 10/27/2019

AHCY activity is intimately connected with folate-mediated one-carbon metabolism, affecting the availability of methyl groups for various methylation reactions.

How is AHCY expression regulated in response to cellular demands? 05/03/2019

AHCY expression can be modulated by transcriptional factors and epigenetic modifications, responding to changes in cellular methylation needs.

How does AHCY interact with other cellular pathways, such as the polyamine biosynthesis pathway? 11/23/2018

AHCY indirectly influences polyamine biosynthesis by providing the methyl groups required for polyamine synthesis.

Describe the structural features of AHCY that contribute to its catalytic efficiency. 09/29/2018

AHCY possesses a TIM-barrel fold with an active site that houses the zinc ion critical for its hydrolytic activity.

Customer Reviews (5)

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Reviews
03/16/2022

    My experiments have never been smoother since I started using this protein.

    10/03/2021

      In our scientific pursuits, this lab protein has surpassed expectations, demonstrating exceptional quality and ease of use.

      01/04/2020

        I highly recommend this protein to fellow researchers. It's a true gem!

        07/11/2019

          We wholeheartedly endorse the lab protein product for its outstanding performance and its significant contribution to our research outcomes.

          12/31/2018

            This protein is like a reliable teammate in the lab – always delivering excellent results.

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