||Recombinant human PRKG1was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=78kDa.
||PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3.
||Sf9 insect cells.
||579 pmoles/min/μg. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 1 mM DTT.
||>12 months at –80°C.