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Recombinant Human PRKG1, His-tagged

Cat.No. : PRKG1-564H
Product Overview : Recombinant human PRKG1was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=78kDa.
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Cat. No. : PRKG1-564H
Description : PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3.
Source : Sf9 insect cells.
Purity : 70%.
Specific Activity : 579 pmoles/min/μg. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Application : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Formulated In : 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 1 mM DTT.
Stability : >12 months at –80°C.
Gene Name : PRKG1 protein kinase, cGMP-dependent, type I [ Homo sapiens ]
Synonyms : PRKG1; protein kinase, cGMP-dependent, type I; PGK; PKG; CGKI; PRKG1B; PRKGR1B; FLJ36117; MGC71944; cGKI-BETA; cGKI-alpha; DKFZp686K042; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; EC 2.7.11.12
Gene ID : 5592
mRNA Refseq : NM_006258.2
Protein Refseq : NP_006249.1
MIM : 176894
UniProt ID : Q13976
Chromosome Location : 10q11.2
Pathway : Gap junction; Long-term depression; Olfactory transduction; Vascular smooth muscle contraction
Function : ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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