Recombinant Human PRKG1, His-tagged
Cat.No. : | PRKG1-564H |
Product Overview : | Recombinant human PRKG1was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=78kDa. |
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- Gene Information
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Cat. No. : | PRKG1-564H |
Description : | PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3. |
Source : | Sf9 insect cells. |
Purity : | 70%. |
Specific Activity : | 579 pmoles/min/μg. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour. |
Application : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Formulated In : | 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 1 mM DTT. |
Stability : | >12 months at –80°C. |
Gene Name : | PRKG1 protein kinase, cGMP-dependent, type I [ Homo sapiens ] |
Synonyms : | PRKG1; protein kinase, cGMP-dependent, type I; PGK; PKG; CGKI; PRKG1B; PRKGR1B; FLJ36117; MGC71944; cGKI-BETA; cGKI-alpha; DKFZp686K042; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; EC 2.7.11.12 |
Gene ID : | 5592 |
mRNA Refseq : | NM_006258.2 |
Protein Refseq : | NP_006249.1 |
MIM : | 176894 |
UniProt ID : | Q13976 |
Chromosome Location : | 10q11.2 |
Pathway : | Gap junction; Long-term depression; Olfactory transduction; Vascular smooth muscle contraction |
Function : | ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity |
Products Types
◆ Recombinant Protein | ||
PRKG1-1767H | Recombinant Human PRKG1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKG1-1102H | Recombinant Human PRKG1 Protein (M1-F686), Tag Free | +Inquiry |
PRKG1-7101M | Recombinant Mouse PRKG1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKG1-1103H | Recombinant Human PRKG1 Protein (M1-F686), GST tagged | +Inquiry |
Prkg1-5125M | Recombinant Mouse Prkg1 Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
PRKG1-2850HCL | Recombinant Human PRKG1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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