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Pea Protein Isolates

Cat.No. : COP14
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Cat. No. : COP14
ProductOverview : SuperProtein Afused with the polyhistidine tag at N-terminus and a single cysteine atC-terminus, was a 246 amino acids protein modified from protein, it wasexpressed in E.Coli.
Description : Super Protein A is aproprietary developed molecule originally modified from recombinantStaphylococcus Aureus protein A with similar function when uesed to makeaffinity beads for Fc fused protein or antibody purification. Super Protein Ais more alkali-stable protein compared with common recombinant protein A,which allow you use up to 0.5M NaOH to do CIP procedure when it has beenconjugated to agarose beads as ligand. There is no significant difference inconjugation procedure between recombinant protein A and Super Protein A,which simplify your process of making better affinity beads by using knowntechnologies and your previous experience on recombinant protein A.
Source : E.coli.
Formulation : Bulk protein in a 0.22 μm filtered solutionof PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally5-8% trehalose and mannitol are added as protectants before lyophilization.
Molecularweight : 26kDa
Endotoxin : Lessthan 0.005 ng endotoxin/1 μg Super Protein A.
Purity : >98%as determined by SDS-PAGE of 6 μg reduced recombinant proteins. All lots aregreater than 95% pure.
Storage : Store at -20℃in lyophilized state after receipt. For long term storage, upon reconstitutionSuper Protein A should be aliquot and store at -20℃ or -80℃. Avoid repeatedfreeze-thaw cycles.
Background :
IntroductionStaphylococcusaureus is a facultative anaerobic Gram-postive coccal bacterium. It isfrequently found as part of the normal skin flora on the skin and nasalpassages. S. aureus is the most commonspecies of staphylococci to cause Staph infections. S. aureus cancause a range of illnesses from minor skin infections,such as pimples, impetigo, boils (furuncles), cellulitis folliculitis, carbuncles, scaldedskin syndrome, and abscesses, to life-threateningdisease such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS), bacteremia, and sepsis.
KeywordsRPS; Super Protein A; Protein A; Super ProteinA

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