|Cat. No. :
||Recombinant HRV-3C Protease contains a Histag, was expressed in E. coli, and purified in vitro usingconventional chromatography techniques.
||HRV His-3C Protease encoded by human rhinovirus 14 is a highly purified recombinantcysteine protease with a His-tag. Substrate recognition and cleavage arelikely to be dependent not only upon primary structural signals, but alsoupon the secondary and tertiary structures of the fusion protein as It hasbeen demonstrated that the enzyme exhibits highest activity around neutral pHat temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at lowtemperature to enhance the stability of the target protein. The catalyticactivity is insensitive to organic solvents (up to 10%); however, it can bestrongly stimulated by high concentration of anions such as sulfate.
||Solution in 50 mM Tris, 150 mM NaCl, 1 mMEDTA, 50% Glycerol, pH 8.0
||One unit will cleave >95% of 100 µgHis•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.
||The enzyme recognizes the cleavage site:Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro.
|10×Cleavage Buffer :
||500mM Tris-HCl, pH-7.0 (at 25℃), 1.5 M NaCl, 10 mM EDTA, 10 mMdithiothreitol. Chill to 5℃ priorto use.
||The high specificity of HRV His-3C proteasemakes it an ideal tool for cleaving fusion proteins at definite cleavagesites. The fusion protein can be purified and cleaved by HRV His-3C to obtainthe target protein. The recombinant HRV His-3C protease is easily removed byIMAC Ni-charged resin.
||Store at -20℃ upon received. Avoid repeated freeze-thawcycles.
|Introduction||Rhinovirus is a species in the genusEnterovirus of the Picornaviridae family of viruses. Thereare 99 recognized types of Human rhinoviruses that differ according to theirsurface proteins. They are lytic in nature and are among the smallest viruses,with diameters of about only 30 nanometers. Other viruses such as smallpox and vaccinia are around 10 times larger at about 300 nanometers.|
|Key words||Turbo3C(HRV 3C) Protease; 3C; HRV-3C|