||Recombinant human SNRPG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Small nuclear ribonucleoprotein polypeptide G, also known as SNRPG, belongs to the snRNPSm proteins family. There are at least seven isoforms, B/B, E, F, G, D1, D2, and D3. This class of common proteins plays an essential role in the biogenesis of the snRNPs. The genes encoding human Sm G map to chromosomes 2. In addition, these proteins represent the major targets for the so-called anti-Sm auto-antibodies which are diagnostic for systemic lupus erythematosus (SLE). One class of these autoantibodies reacts specifically with native Sm E-F-G complexes.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.1M NaCl
||10.6 kDa (96aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNLVIDECVEMAT SGQQNNIGMV VIRGNSIIMLEALERV
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.