Recombinant Human RIPK3, GST-tagged, Active

• Cat.No.: RIPK3-2758H
• Product Overview: Recombinant Human RIPK3 encoding full-lengthhuman RIPK3 produced by baculovirus in Sf9 insect cells, was fused a GST-tag at N-terminal.

Specification

• Cat. No.: RIPK3-2758H
• Description: RIPK3 is a member of the receptor-interactingprotein (RIP) family of serine/threonine protein kinases, which contains aC-terminal domain unique from other RIP family members. RIPK3 ispredominantly localized to the cytoplasm, and specifically, are colocalizedin the mitochondrion which can undergo nucleocytoplasmic shuttlingdependent on novel nuclear localization and export signals. RIPK3 is acomponent of the tumor necrosis factor (TNF) receptor-I signaling complex, andcan induce apoptosis and weakly activate the NF-kappaB transcription factorand caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis byfunctioning in a proteolytically active complex with CFLAR and that thiscomplex is required for the protective function.
• Source: Baculovirus
• MolecularWeight: 96 kDa
• Formulation: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mMNaCl, 10mM glutathione, 0.1mM EDTA, 0,25mM DTT, 0.1mM PMSF, 25% glycerol.
• Purity: >70%
• SpecificActivity: 38nM/min/mg
• Concentration: 0.05μg / μl
• Applications: Kinase Assay, Western Blot
• Storage: Storeproduct at –70°C. For optimal storage, aliquot target intosmaller quantities after centrifugation and store at recommended temperature.For most favorable performance, avoid repeated handling and multiplefreeze/thaw cycles.

Gene Information

• Gene Name: RIPK3receptor-interacting serine-threonine kinase 3 [ Homo sapiens ]
• Official Symbol: RIPK3
• Synonyms: RIPK3;receptor-interacting serine-threonine kinase 3; receptor-interactingserine/threonine-protein kinase 3; RIP3; RIP-3; RIP-like protein kinase 3; receptorinteracting protein 3; receptor-interacting protein 3; OTTHUMP00000164791; EC2.7.11.1
• Gene ID: 11035
• mRNA Refseq: NM_006871
• Protein Refseq: NP_006862
• MIM: 605817
• UniProt ID: Q9Y572
• Chromosome Location: 14q12
• Pathway: CytosolicDNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensingpathway, conserved biosystem; Immune System, organism-specific biosystem;Innate Immune System, organism-specific biosystem; TNF-alpha/NF-kB SignalingPathway, organism-specific biosystem
• Function: ATP binding;NF-kappaB-inducing kinase activity; nucleotide binding; protein binding;protein kinase activity