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Mouse Anti-Human Carboxypeptidase B1 Monoclonal Antibody

Cat.No.: CAB11512MH
Product Overview: Mouse Monoclonal Antibody to Human Carboxypeptidase B1
Antigen Description: Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Specificity: Human CPB1 / PCPB. No cross-reactivity in WB and ELISA with mouse CPB1, human CPA1, human CPA2, human CPE and human cell lysate (293 cell line)
Immunogen: Recombinant human CPB1 protein
Host: Mouse
Isotype: Mouse IgG1
Species: Human
Clone: 3D11D7C13
Applications: WB; ELISA
Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CPB1 in WB. Using a DAB detection system, the detection limit for human CPB1 is approximately 1 ng/lane under non-reducing conditions and reducing conditionsELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CPB1. The detection limit for human CPB1 is 0.31 ng/well
Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CPB1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: CPB1 carboxypeptidase B1 (tissue) [ Homo sapiens ]
Official Symbol: CPB1
Synonyms: CPB1; carboxypeptidase B1 (tissue); carboxypeptidase B; pancreatic carboxypeptidase B; protaminase; tissue carboxypeptidase B; procarboxypeptidase B; pancreas-specific protein; CPB; PASP; PCPB; DKFZp779K1333;
Gene ID: 1360
mRNA Refseq: NM_001871
Protein Refseq: NP_001862
MIM: 114852
UniProt ID: P15086
Chromosome Location: 3q24
Pathway: Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem;
Function: carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding;

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