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Mouse Anti-Human Carboxypeptidase B1 Monoclonal Antibody

Cat.No. : CAB11512MH
Product Overview : Mouse Monoclonal Antibody to Human Carboxypeptidase B1
  • Specification
  • Gene Information
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Antigen Description : Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Specificity : Human CPB1 / PCPB. No cross-reactivity in WB and ELISA with mouse CPB1, human CPA1, human CPA2, human CPE and human cell lysate (293 cell line)
Immunogen : Recombinant human CPB1 protein
Host : Mouse
Isotype : Mouse IgG1
Species : Human
Clone : 3D11D7C13
Applications : WB; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CPB1 in WB. Using a DAB detection system, the detection limit for human CPB1 is approximately 1 ng/lane under non-reducing conditions and reducing conditionsELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CPB1. The detection limit for human CPB1 is 0.31 ng/well
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CPB1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CPB1 carboxypeptidase B1 (tissue) [ Homo sapiens ]
Official Symbol : CPB1
Synonyms : CPB1; carboxypeptidase B1 (tissue); carboxypeptidase B; pancreatic carboxypeptidase B; protaminase; tissue carboxypeptidase B; procarboxypeptidase B; pancreas-specific protein; CPB; PASP; PCPB; DKFZp779K1333;
Gene ID : 1360
mRNA Refseq : NM_001871
Protein Refseq : NP_001862
MIM : 114852
UniProt ID : P15086
Chromosome Location : 3q24
Pathway : Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem;
Function : carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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