Active Recombinant Human PHKG1, GST-tagged

• Cat.No.: PHKG1-1448H
• Product Overview: Recombinant full-length human PHKG1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PHKG1 or phosphorylase kinase gamma 1 is a member of the Ser/Thr protein kinase family that encodes a protein with one protein kinase domain and two calmodulin-binding domains. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme.PHKG1 is the catalytic member of a 16-subunit protein kinase complex that contains equimolar ratios of 4 subunit types known as alpha, beta, gamma and delta. Skeletal muscle contains the highest amount of phosphorylase kinase enzymatic activity, although activity is also observed in liver, cardiac muscle, brain, and several other tissues.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 29 nmol/min/mg
• Molecular Mass: ~70 kDa
• Protein length: Full length
• Purity: >75%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PHKG1 phosphorylase kinase, gamma 1 (muscle) [ Homo sapiens ]
• Official Symbol: PHKG1
• Synonyms: PHKG1; phosphorylase kinase, gamma 1 (muscle); PHKG; phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform; phosphorylase kinase gamma; phosphorylase kinase subunit gamma-1
• Gene ID: 5260
• mRNA Refseq: NM_006213
• Protein Refseq: NP_006204
• MIM: 172470
• UniProt ID: Q16816
• Chromosome Location: 7p11.2
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem
• Function: ATP binding; calmodulin binding; enzyme binding; nucleotide binding; phosphorylase kinase activity; protein serine/threonine kinase activity