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Recombinant Human MIF

Cat.No. : MIF-09H
Product Overview : Recombinant Human MIF was expressed in E.coll.
Description : This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Source : E.coll
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Molecular Mass : Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids.
AA Sequence : MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALCSLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
Endotoxin : Less than 1EU/µg of rHuMIF as determined by LAL method.
Purity : > 97 % by SDS-PAGE and HPLC analyses
Storage : This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Gene Name : MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) [ Homo sapiens (human) ]
Official Symbol : MIF
Synonyms : MIF; macrophage migration inhibitory factor; GIF; GLIF; MMIF; phenylpyruvate tautomerase; glycosylation-inhibiting factor; EC 5.3.2.1; Glycosylation-inhibiting factor; Phenylpyruvate tautomerase
Gene ID : 4282
mRNA Refseq : NM_002415
Protein Refseq : NP_002406
MIM : 153620
UniProt ID : P14174
Chromosome Location : 22q11.23
Pathway : Adipogenesis; Phenylalanine metabolism; Tyrosine metabolism
Function : cytokine activity; dopachrome isomerase activity; phenylpyruvate tautomerase activity; protein binding

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