||This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis.
||Saporin is a ribosome-inactivating protein (RIP) of type I. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins.
||Upon introduction into the cells, saporin will arrest the protein synthesis by damaging the ribosmes.
||95% by SDS-PAGE and Western Blot.
||Aliquot and keep at -20°C for long-term storage. For short term keep at 4°C. Avoid repetitive freeze/thaw cycles.
||Saporin may be reconstituted in the buffer of choice for example PBS.