Recombinant Human CDC34, His-tagged
Cat.No. : | CDC34-224H |
Product Overview : | Recombinant full length human UBE2R1 was expressed in E. coli cells using an N-terminal His tag. |
- Specification
- Gene Information
- Related Products
Description : | UBE2R1 is a member of the ubiquitin-conjugating enzyme family which catalyzes the covalent attachment of ubiquitin to other proteins. UBE2R1 is a part of the large multiprotein complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators and for the initiation of DNA replication. CK2 can phosphorylate CDC34 and UBC3B at corresponding serine residue in the C terminus of each protein. Phosphorylated UBC3B and CDC34 binds specifically to the F-box protein beta-TRCP which results in enhanced degradation of beta-catenin, a substrate of BTRC. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
Molecular Mass : | ~33 kDa |
Purity : | >75% by densitometry |
Applications : | Western Blot |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1 μg/μl |
Gene Name : | CDC34 cell division cycle 34 homolog (S. cerevisiae) [ Homo sapiens ] |
Official Symbol : | CDC34 |
Synonyms : | CDC34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; ubiquitin-conjugating enzyme E2 R1; E2 CDC34; UBC3; UBE2R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing; UBCH3; E2-CDC34; |
Gene ID : | 997 |
mRNA Refseq : | NM_004359 |
Protein Refseq : | NP_004350 |
MIM : | 116948 |
UniProt ID : | P49427 |
Chromosome Location : | 19p13.3 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; |
Function : | ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity; |
Products Types
◆ Recombinant Protein | ||
CDC34-0036H | Recombinant Human CDC34 Protein (A2-S236), Tag Free | +Inquiry |
Cdc34-2074M | Recombinant Mouse Cdc34 Protein, Myc/DDK-tagged | +Inquiry |
CDC34-0929H | Recombinant Human CDC34 Protein, GST-Tagged | +Inquiry |
CDC34-0037H | Recombinant Human CDC34 Protein (A2-S236), His/Strep tagged | +Inquiry |
CDC34-138H | Recombinant Human CDC34, His-tagged | +Inquiry |
◆ Lysates | ||
CDC34-7660HCL | Recombinant Human CDC34 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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