||Recombinant human YSK4 (MAP3K19) (941-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
||YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat. The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring in the large-conductance Ca2+-activated K+ channels. The YSK4 kinase function has not been made clearly yet.
||Sf9 insect cells using baculovirus
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
||Kinase Assay, Western Blot
||Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.