"Ni" Related Products

Ni Agarose high flow

Cat.no: Ni-001A
Product Overview: Ni Agarose is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.
Characteristics: Ni Agarose High Flow charged with nickel ions will selectively retain proteins containing a histidine-tag. Histidinetagged protein is then eluted using buffers containing imidazole. For purification of insoluble histidine-tagged proteins (fusion proteins expressed as inclusion bodies) requiring denaturing conditions, up to 8 M urea or 6 M guanidine hydrochloride can be used in the buffers to solubilise insoluble proteins.
Size: 10 mL, 25 mL, 500 mL
Matrix: 4% cross-linked agarose
Average particle size: 90 μm
Ligand: Ni2+
Dynamic binding capacity: 20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin
Recommended column height: 5‐20cm
Temperature Stability: 4‐40 °C
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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