Recombinant Human ARSK 293 Cell Lysate
Cat.No. : | ARSK-8673HCL |
- Specification
- Gene Information
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Description : | Antigen standard for arylsulfatase family, member K (ARSK) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ARSK arylsulfatase family, member K [ Homo sapiens ] |
Official Symbol : | ARSK |
Synonyms : | ARSK; arylsulfatase family, member K; arylsulfatase K; DKFZp313G1735; TSULF; ASK; telethon sulfatase; |
Gene ID : | 153642 |
mRNA Refseq : | NM_198150 |
Protein Refseq : | NP_937793 |
MIM : | 610011 |
UniProt ID : | Q6UWY0 |
Chromosome Location : | 5q15 |
Function : | arylsulfatase activity; hydrolase activity; metal ion binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe ARSK protein is known to hydrolyze a specific class of sulfates known as sulfatides. Sulfatides are a type of glycosphingolipid found in various tissues and cells, particularly in the nervous system. ARSK catalyzes the breakdown of sulfatides into sulfate ions and ceramide.
Yes, genetic variations or mutations in the ARSK gene have been reported in individuals with ARSK-related disorders. These variations can interfere with the proper functioning of the enzyme, leading to impaired sulfate metabolism and the associated clinical symptoms.
Deficiency or dysfunction of the ARSK protein can lead to a rare genetic disorder called ARSK-related disorder. This condition is characterized by the accumulation of sulfatides, a type of lipid, in various organs and tissues, leading to tissue damage and dysfunction.
Some studies have suggested that ARSK dysfunction and subsequent sulfatide accumulation may contribute to the pathogenesis of certain neurodegenerative diseases, such as multiple sclerosis and metachromatic leukodystrophy. The deposition of sulfatides in neural tissues may disrupt myelin integrity and impair nerve conduction.
Currently, there are no specific treatments or therapies available for ARSK-related disorders. Management typically involves symptomatic and supportive care to address the specific symptoms and complications that arise due to sulfatide accumulation.
Yes, genetic testing can be used to diagnose ARSK-related disorders. By sequencing the ARSK gene and identifying disease-causing variations or mutations, a definitive diagnosis can be made. Genetic counseling may also be offered to affected individuals and their families.
The long-term effects of ARSK dysfunction may vary depending on the severity and specific manifestations of the disorder. Sulfatide accumulation can lead to damage in various organs, including the brain, liver, and kidneys, resulting in neurological, hepatic, or renal impairments over time.
Currently, there is limited research on whether the ARSK protein is implicated in other diseases or conditions. Further studies are needed to determine if it plays a role in other aspects of sulfate metabolism or if dysregulation of ARSK is associated with other disorders.
At present, no specific inhibitors or activators of the ARSK protein have been identified. The development of small molecules or compounds that can modulate ARSK activity could hold potential for future therapeutic interventions, but more research is needed in this area.
The regulation of the ARSK protein is not fully understood. It is likely controlled at the transcriptional level, where the expression of the ARSK gene is influenced by various regulatory factors. Additionally, post-translational modifications and protein-protein interactions may also participate in the regulation of ARSK activity.
Customer Reviews (3)
Write a reviewWhen used in Western Blotting experiments, the ARSK protein consistently generates clear and distinct protein bands.
It has been invaluable in protein electron microscopy structure analysis, facilitating detailed investigations and yielding valuable insights.
This exceptional clarity allows for accurate quantification and analysis, contributing to the overall success of my research endeavors.
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