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Recombinant Human AGBL2 293 Cell Lysate

Cat.No. : AGBL2-8983HCL
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  • Gene Information
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Description : Antigen standard for ATP/GTP binding protein-like 2 (AGBL2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AGBL2 ATP/GTP binding protein-like 2 [ Homo sapiens ]
Official Symbol : AGBL2
Synonyms : AGBL2; ATP/GTP binding protein-like 2; cytosolic carboxypeptidase 2; FLJ23598;
Gene ID : 79841
mRNA Refseq : NM_024783
Protein Refseq : NP_079059
UniProt ID : Q5U5Z8
Chromosome Location : 11p11.2
Function : metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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Can external factors regulate AGBL2 levels? 07/28/2022

Yes, AGBL2 expression and activity can be influenced by cellular cues.

Does AGBL2 exhibit enzymatic behavior? 11/20/2021

AGBL2 displays metalloprotease activity, enabling protein peptide bond cleavage.

What roles does AGBL2 play in cells? 07/23/2021

AGBL2 has implications in diverse cellular processes, including possible protein regulation.

Does AGBL2 contribute to autophagy? 06/29/2021

AGBL2's connection to autophagy, cellular self-degradation, is under scrutiny.

Are AGBL2 variations tied to diseases? 06/19/2021

Some evidence suggests AGBL2 mutations could be linked to specific neurodegenerative conditions.

How does AGBL2 compare to other binding proteins? 05/28/2021

AGBL2's unique structure and roles distinguish it from other binding proteins.

Is AGBL2 part of signaling pathways? 04/08/2021

Research is ongoing to identify AGBL2's involvement in different cellular signaling networks.

Does AGBL2 appear in multiple cell types? 08/17/2020

Yes, AGBL2's presence spans various cell types, indicating widespread involvement.

Where is AGBL2 primarily situated? 07/06/2020

AGBL2 is localized within the cell's cytoplasm, actively fulfilling its functions.

How does AGBL2 respond to cellular stress? 07/05/2019

AGBL2's response to stress is a fascinating area under exploration.

Could AGBL2 influence protein quality control? 03/13/2019

AGBL2's functions could contribute to maintaining proper protein quality in cells.

What defines the AGBL2 protein? 10/27/2018

AGBL2 refers to "ATP/GTP-binding protein-like 2," known for its varied cellular functions.

Are animal models used for AGBL2 study? 06/23/2018

Animal models aid in understanding AGBL2's functions and implications.

How does AGBL2 impact cellular balance? 05/23/2018

AGBL2's functions likely contribute to maintaining cellular equilibrium.

Is AGBL2's regulatory role well-defined? 03/13/2018

While tied to protein processes, AGBL2's full regulatory roles continue to unfold.

What roles does AGBL2 play in cells? 03/09/2018

AGBL2 has implications in diverse cellular processes, including possible protein regulation.

What's notable about AGBL2's 3D structure? 02/13/2018

AGBL2's structure reveals domains shedding light on its potential roles.

What defines the AGBL2 protein? 02/05/2018

AGBL2 refers to "ATP/GTP-binding protein-like 2," known for its varied cellular functions.

Is AGBL2 crucial for normal development? 08/15/2017

AGBL2's roles in development are being unveiled, suggesting cellular contributions.

Is AGBL2's structure well-explored? 05/24/2017

Yes, AGBL2's structure features functional domains that hint at its cellular roles.

Customer Reviews (5)

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Reviews
10/09/2022

     Replicating results confirmed the product’s reliability and significance in our research.

    03/03/2019

      Using this product, we achieved consistent and clean co-immunoprecipitation results.

      03/27/2018

        Protein AGBL2 consistently inhibited the formation of angiogenic tubules.

        10/09/2017

           The product’s cost-effectiveness and clear instructions were valuable in our experiments.

          08/09/2016

            Protein reliably induced consistent morphological changes in cells.

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