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Recombinant Human AES 293 Cell Lysate

Cat.No. : AES-8991HCL
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  • Gene Information
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Description : Antigen standard for amino-terminal enhancer of split (AES), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AES amino-terminal enhancer of split [ Homo sapiens ]
Official Symbol : AES
Synonyms : AES; amino-terminal enhancer of split; GRG5; TLE5; gp130-associated protein GAM; GRG; ESP1; AES-1; AES-2;
Gene ID : 166
mRNA Refseq : NM_001130
Protein Refseq : NP_001121
MIM : 600188
UniProt ID : Q08117
Chromosome Location : 19p13.3
Pathway : Androgen Receptor Signaling Pathway, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem;
Function : protein binding; transcription corepressor activity; transcription corepressor activity; transcription corepressor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Are there any known post-translational modifications or regulatory factors that modulate the activity or stability of AES protein? 06/28/2022

Post-translational modifications, such as phosphorylation or acetylation, and regulatory factors can modulate the activity or stability of AES protein, influencing its function in gene regulation and cellular processes.

What experimental techniques or assays have been employed to study the functional significance of AES protein, such as gene expression analysis or chromatin immunoprecipitation? 12/27/2021

Experimental techniques such as gene expression analysis, chromatin immunoprecipitation, or functional genomics approaches have been employed to investigate the functional significance of AES protein in gene regulation and cellular processes.

Can AES protein be targeted or manipulated pharmacologically to modulate cellular processes or potentially serve as a therapeutic target for diseases associated with dysregulated gene expression or abnormal cell growth? 11/09/2021

AES protein could be a potential pharmacological target to modulate gene expression, cellular processes, or serve as a therapeutic target for diseases associated with dysregulated gene expression or abnormal cell growth. Further research is needed to explore its therapeutic potential and develop targeted interventions.

How does dysregulation or dysfunction of AES protein affect cellular functions, developmental processes, or disease development? 07/27/2021

Dysregulation or dysfunction of AES protein can disrupt cellular functions, developmental processes, and contribute to disease development, such as cancer, neurodegenerative disorders, or developmental disorders.

What are the cellular and subcellular localization patterns of AES protein, and how are they determined experimentally? 06/19/2021

The cellular and subcellular localization patterns of AES protein can vary across different cell types and developmental stages. Experimental techniques such as immunofluorescence microscopy or subcellular fractionation can be used to determine its localization.

What are the transcriptional regulatory mechanisms that control the expression of AES protein in different cell types or under specific physiological conditions? 03/03/2019

The expression of AES protein is regulated at the transcriptional level by specific promoters and transcription factors. Cellular signaling pathways and environmental cues can influence its expression in a context-dependent manner.

Are there any genetic variations or mutations in the AES gene that impact the expression or function of AES protein, and what are the implications for cellular processes? 11/22/2018

Genetic variations or mutations in the AES gene may impact the expression or function of AES protein, potentially leading to dysregulated gene expression, altered cellular processes, or disease susceptibility.

Does AES protein interact with specific molecules or participate in protein complexes, and what are the functional consequences of these interactions? 10/24/2018

AES protein may interact with specific molecules, such as transcription factors or chromatin modifiers, forming protein complexes that modulate gene expression and cellular processes.

How does AES protein modulate gene expression and transcriptional activity, and what are the underlying molecular mechanisms? 09/19/2018

AES protein modulates gene expression and transcriptional activity by interacting with transcriptional regulators, co-repressors, or chromatin remodeling complexes, potentially leading to transcriptional repression or modulation of target genes.

What is the role of AES protein in cellular processes such as cell proliferation, differentiation, or apoptosis, and how is this function assessed experimentally? 08/24/2018

AES protein plays a role in cellular processes such as cell proliferation, differentiation, or apoptosis, potentially through its interactions with transcription factors or modulation of gene expression. Experimental approaches including loss-of-function studies or functional assays can assess its function.

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09/12/2022

    Understanding protein-protein interaction networks in oxidative stress responses.

    06/15/2022

      Detecting protein-protein interactions in ion transport and homeostasis.

      07/27/2019

        Exploring protein-protein interactions involved in cytoskeleton organization.

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