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Recombinant Human ADPRHL1 293 Cell Lysate

Cat.No. : ADPRHL1-9002HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosylhydrolase like 1 (ADPRHL1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ADPRHL1 ADP-ribosylhydrolase like 1 [ Homo sapiens ]
Official Symbol : ADPRHL1
Synonyms : ADPRHL1; ADP-ribosylhydrolase like 1; [Protein ADP-ribosylarginine] hydrolase-like protein 1; ARH2; ADP-ribosyl-hydrolase; ADP-ribosylhydrolase 2;
Gene ID : 113622
mRNA Refseq : NM_138430
Protein Refseq : NP_612439
MIM : 610620
UniProt ID : Q8NDY3
Chromosome Location : 13q34
Function : ADP-ribosylarginine hydrolase activity; hydrolase activity; magnesium ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (9)

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How are ADPRHL1 proteins implicated in cancer? 07/23/2021

ADPRHL1 has been found to be overexpressed in several types of cancer cells, such as colon, breast, and lung cancer cells. Studies suggest that ADPRHL1 may promote cancer cell survival by modulating signaling pathways that regulate cell growth and apoptosis. Inhibition of ADPRHL1 has been shown to reduce cancer cell growth and sensitize cancer cells to chemotherapy, suggesting that ADPRHL1 may be a potential therapeutic target for cancer treatment.

Are there any genetic variants of ADPRHL1 associated with disease? 05/12/2021

Yes, there have been several genetic variants of ADPRHL1 identified that are associated with disease. For example, a single nucleotide polymorphism (SNP) in the ADPRHL1 gene has been linked to an increased risk of developing prostate cancer. Another SNP has been associated with an increased risk of developing schizophrenia. However, more research is needed to fully understand the links between ADPRHL1 genetic variants and disease.

Can ADPRHL1 proteins be used as a biomarker for disease? 11/12/2019

There is some evidence to suggest that ADPRHL1 proteins may be useful as a biomarker for certain diseases. For example, elevated levels of ADPRHL1 have been found in the blood and cerebrospinal fluid of patients with Alzheimer's disease and Parkinson's disease, respectively. Additionally, ADPRHL1 has been proposed as a potential biomarker for early detection of breast cancer. However, further research is needed to confirm the clinical utility of ADPRHL1 as a biomarker for these and other diseases.

What other areas of medical research are exploring the role of ADPRHL1? 09/15/2019

In addition to cancer, ADPRHL1 has been implicated in several other areas of medical research. For example, studies have suggested that ADPRHL1 plays a role in the regulation of glucose metabolism, and may be involved in the development of type 2 diabetes. Additionally, ADPRHL1 has been implicated in the regulation of inflammatory responses, and may be involved in the pathogenesis of autoimmune diseases such as rheumatoid arthritis and lupus. Further research is needed to fully understand the role of ADPRHL1 in these and other areas of medical research.

Are there any known genetic variants of the ADPRHL1 gene? 04/19/2019

Yes, several genetic variants of the ADPRHL1 gene have been identified. These include single nucleotide polymorphisms (SNPs) that affect the expression and activity of the enzyme. For example, one study found that a common SNP in the ADPRHL1 gene is associated with an increased risk of breast cancer in women. Other studies have identified SNPs that are associated with the risk of developing type 2 diabetes and other metabolic disorders. Additionally, mutations in the ADPRHL1 gene have been linked to hereditary spastic paraplegia. These genetic variants may have important implications for disease risk and personalized medicine.

Are there any inhibitors of ADPRHL1 that have been identified? 10/10/2018

Yes, several compounds have been identified that inhibit the activity of ADPRHL1. One of the most potent inhibitors is 2-palmitoylisopropyl phosphoramidate (2-PIPA), which inhibits ADPRHL1 by covalently modifying a catalytic residue in the enzyme's active site. In addition, several other compounds have been identified through high-throughput screening and structure-based drug design approaches. These compounds often target specific structural features of the enzyme, such as the active site residues or the C-terminal domain, and may have potential therapeutic applications in the treatment of diseases such as Alzheimer's and Parkinson's.

How are ADPRHL1 protein levels measured in clinical settings? 03/20/2018

ADPRHL1 protein levels can be measured in clinical settings using various laboratory techniques, such as ELISA or Western blotting. These techniques involve collecting a sample of blood, urine, or tissue from the patient, and then measuring the amount of ADPRHL1 protein present in the sample. These measurements can provide valuable information about the patient's health status and disease progression, and may be used to inform treatment decisions. However, these techniques are not routinely used in clinical practice and further research is needed to establish their clinical utility.

Are there any drugs that target ADPRHL1? 12/23/2017

There are currently no drugs approved for the specific targeting of ADPRHL1. However, several studies have investigated the efficacy of ADPRHL1 inhibition as a potential cancer therapy. One study found that a small molecule inhibitor of ADPRHL1 reduced the growth of breast cancer cells in vitro and in vivo. Additionally, another study found that inhibiting ADPRHL1 enhanced the efficacy of chemotherapy in colorectal cancer cells. These findings suggest that ADPRHL1 inhibition may be a promising therapeutic strategy for cancer, but further research is needed to develop effective drugs that specifically target ADPRHL1.

Is ADPRHL1 implicated in any neurological diseases? 11/05/2016

Yes, ADPRHL1 has been implicated in several neurological diseases. Studies have found that the expression of ADPRHL1 is increased in the brains of patients with Alzheimer's disease and that ADPRHL1 may contribute to the development of the disease by promoting the accumulation of beta-amyloid. ADPRHL1 has also been proposed as a potential therapeutic target for Parkinson's disease, as its inhibition may help to prevent the loss of dopaminergic neurons in the brain. Additionally, mutations in the ADPRHL1 gene have been linked to hereditary spastic paraplegia, a rare neurodegenerative disorder that affects the lower limbs and causes progressive muscle weakness and spasticity.

Customer Reviews (5)

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Reviews
12/09/2022

    Since I needed a high purity protein, the manufacturer recommended ADPRHL1 based on its purity, and it did not disappoint.

    06/04/2022

      Their attention to detail and responsiveness have made them a trusted partner in my scientific endeavors.

      10/25/2021

        The ADPRHL1 protein I have acquired is of exceptional quality and meets all of my experimental requirements.

        09/04/2019

          I am pleased with the excellent technical support provided by the manufacturer, which has helped me to overcome any technical issues and carry out my research with ease.

          09/30/2018

            I am very satisfied with the product and the support provided by the manufacturer, and I highly recommend them to other researchers in the field.

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