PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

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PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

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Cat.No. : PC-12-2144R
Description : Whole cell lysate (denatured)
Species : Rat
Tissue Type : Adrenal Gland
Preparation method : Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Size : 200 ug
Recommended Usage : The lysate is ready to load on SDS-PAGE for Western blotting.
Storage Buffer : In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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