Active Native Bovine glu-Plasminogen

• Cat.No.: PLG-268B

Specification

• Description: Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 μM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and prothrombin. Plasminogen contains one high affinity (Kd=9x10-6M) and four low affinity (Kd=5x10-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and α2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr=83,000) by plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen.
• Source: Plasma
• Species: Bovine
• Form: 50% (vol/vol) glycerol/H2O
• Bio-activity: < 10 ppm plasmin activity for>
• Purity: >95% by SDS-PAGE. NOT tissue/cell culture grade. Not tested for endotoxin.
• Characteristic: Extinction coefficient: 17.0, Isoelectric point:6.2, Percent carbohydrate: Approximately 2%, Structure:single chain, 24 intra chain disulfide bridges, 5 kringle regions
• Storage: -20°C

Gene Information

• Gene Name: PLG plasminogen [ Bos taurus ]
• Official Symbol: PLG
• Synonyms: LPA; plasminogen; plasmin; lipoprotein, Lp(a)
• Gene ID: 280897
• mRNA Refseq: NM_173951
• Protein Refseq: NP_776376
• UniProt ID: P06868
• Chromosome Location: chromosome: 9
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem
• Function: protein binding; serine-type endopeptidase activity