Recombinant RFP-rProtein A, His-tagged


Home / Products / Fluorescent Proteins / Recombinant RFP-rProtein A, His-tagged

Recombinant RFP-rProtein A, His-tagged

RFP Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : RFP-28H
Product Overview : Recombinant red fluorescence Protein (RFP)-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). RFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and thereforeare ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.
Source : E. coli
Tag : His
Form : Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.
Bio-activity : IgG Binding: >95%. Under optimal conditions, 1 mg RFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of EGFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
Endotoxin : < 0.05>
Purity : >98% by SDS-PAGE and HPLC
Applications : The protein is suitable as control reagent for RFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 557 nm and emission wavelength at 585 nm.
Storage : Store at -20 centigrade. -80 centigrade for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.

Products Types

◆ Recombinant Protein

RFP-005ERecombinant Red Fluorescent Protein, His-tagged+Inquiry

See All RFP Recombinant Protein

Related Gene


Online Inquiry

  • Note: There will be extra charge for optional service!
  • Verification Code Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy