Active Recombinant Mouse Gdf9

• Cat.No.: Gdf9-180M
• Product Overview: Recombinant Mouse Gdf9 (Accession # AAA53035) Gly307-Arg441 was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Predicted N Terminal: Gly307
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.100 ng/mL rmGDF-9 can effectively induce Smad2 phosphorylation.
• Molecular Mass: Recombinant Mouse Gdf9 has a calculated MW of 15.6 kDa. In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing 0.1% bovine serum albumin.

Gene Information

• Gene Name: Gdf9 growth differentiation factor 9 [ Mus musculus ]
• Official Symbol: Gdf9
• Synonyms: GDF9; growth differentiation factor 9; growth/differentiation factor 9; Gdf-9
• Gene ID: 14566
• mRNA Refseq: NM_008110
• Protein Refseq: NP_032136