LL/2 (mouse Lewis lung carcinoma) whole cell lysate LL-2-991M

Cat.No. :	LL-2-991M
Species :	Mouse
Tissue Type :	Lung
Preparation method :	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Lysis buffer :	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Size :	200 ug
Recommended Usage :	Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer :	In modified RIPA Lysis Buffer.
Storage Instruction :	Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Applications :	Western Blot;Immunoprecipitation;

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