Nickel Beads

• Cat.No.: Nickel-31A
• Product Overview: Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted.

Specification

• Tag: Non
• Cat.no: Nickel-31A
• Matrix: Highly cross-linked spherical agarose, 4%
• Average particle size: 40‐165 μm
• Dynamic binding capacity: ~6-9 mg pure (His) 6 -tagged protein (Mr ~ 49 000) per ml
• Recommended flow rate: 1-2 ml/min/ cm2
• pH working range: 2-11
• Elution buffer: 0.02 M sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4
• Storage: Storage conditions: equilibrate the column with the Binding buffer added with NaN3 0.1% (w/v) or 20% ethanol as preservatives.