"Nickel" Related Products

      Nickel Beads

      Cat.no : Nickel-31A
      Product Overview : Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted.
      Matrix : Highly cross-linked spherical agarose, 4%
      Average particle size : 40‐165 μm
      Dynamic binding capacity : ~6-9 mg pure (His) 6 -tagged protein (Mr ~ 49 000) per ml
      Recommended flow rate : 1-2 ml/min/ cm2
      pH working range : 2-11
      Elution buffer : 0.02 M sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4
      Storage : Storage conditions: equilibrate the column with the Binding buffer added with NaN3 0.1% (w/v) or 20% ethanol as preservatives.
      Products Types

      ◆ Assay kits

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