||Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted.
||Highly cross-linked spherical agarose, 4%
|Average particle size:
|Dynamic binding capacity:
||~6-9 mg pure (His) 6 -tagged protein (Mr ~ 49 000) per ml
|Recommended flow rate:
||1-2 ml/min/ cm2
|pH working range:
||0.02 M sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4
||Storage conditions: equilibrate the column with the Binding buffer added with NaN3 0.1% (w/v) or 20% ethanol as preservatives.