"Nickel" Related Products

Nickel Beads

Cat.no: Nickel-31A
Product Overview: Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted.
Matrix: Highly cross-linked spherical agarose, 4%
Average particle size: 40‐165 μm
Dynamic binding capacity: ~6-9 mg pure (His) 6 -tagged protein (Mr ~ 49 000) per ml
Recommended flow rate: 1-2 ml/min/ cm2
pH working range: 2-11
Elution buffer: 0.02 M sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4
Storage: Storage conditions: equilibrate the column with the Binding buffer added with NaN3 0.1% (w/v) or 20% ethanol as preservatives.
Products Types

◆ Assay kits

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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