Cat. No. : |
GHR-450H |
Description : |
Human growth hormone receptor (GH R) is a type I membrane protein and is a member of the class I cytokine receptor family. GH R is highly expressed in the liver and in skeletal muscle as well as in the kidney, bladder, adrenal gland, brain, bone, cartilage, lung and stomach. Activation of GH R by GH regulates an extensive variety of physiological functions relating to human growth and metabolism. Specifically, binding of GH to GH R promotes the growth of bone, cartilage, and soft tissues, which occurs maximally during puberty. GH R activation also enhances the levels of hormones and neurotransmitters in the CNS, including IGF-1 and somatostatin. |
Source : |
Human 293 cells. |
Amino Acid Sequence : |
FSGSEATAAILSRAPWSLQSVNPGL KTNSSKEPKFTKCRSPERETFSCHW TDEVHHGTKNLGPIQLFYTRRNTQE WTQEWKECPDYVSAGENSCYFNSSF TSIWIPYCIKLTSNGGTVDEKCFSV DEIVQPDPPIALNWTLLNVSLTGIH ADIQVRWEAPRNADIQKGWMVLEYE LQYKEVNETKWKMMDPILTTSVPVY SLKVDKEYEVRVRSKQRNSGNYGEF SEVLYVTLPQMRIPKVDKKVEPKSC DKTHTCPPCPAPELLGGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHED PEVKFNWYVDGVEVHNAKTKPREEQ YNSTYRVVSVLTVLHQDWLNGKEYK CKVSNKALPAPIEKTISKAKGQPRE PQVYTLPPSRDELTKNQVSLTCLVK GFYPSDIAVEWESNGQPENNYKTTP PVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSP GK. |
Molecular Mass : |
Growth Hormone Receptor-Fc Chimera migrates as a broad band between 65 and 85 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. |
pI : |
Growth Hormone Receptor-Fc Chimera separates into a number of isoforms with a pI between 5.0 and 7.7 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified GH R-Fc Chimera that has a predicted pI of 6.90. |
% Carbohydrate : |
Purified Growth Hormone Receptor-Fc Chimera consists of 15-35% carbohydrate by weight. |
Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |