| Cat. No. : |
Scarb2-851M |
| Description : |
Plasminogen activator, urokinase, also known as PLAU and UPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. |
| Molecular Mass : |
The secreted recombinant human PLAU comprises 422 amino acids with a predicted molecular mass of 46 kDa. As a result of glycosylation, rhPLAU migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length PLAU with the molecular mass of 18, 32 and 50 kDa respectively in SDS-PAGE under reducing conditions. |
| Source : |
Human Cells (HEK293). |
| Predicted N Terminal : |
Ser 21. |
| Endotoxin : |
< 1.0 EU per 1µg cytokine as determined by the LAL method. |
| Formulation : |
Supplied as a 0.2µm filtered solution of PBS, pH7.4. |
| Reconstitution : |
Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Purity : |
> 97%, as determined by SDS-PAGE. |
| Stability : |
Samples are stable for up to twelve months from date of receipt -70°C. |
| Storage : |
Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles. |
