Active Recombinant Human PSIP1, 322-530aa

• Cat.No.: PSIP1-1079H

Specification

• Cat. No.: PSIP1-1078H
• Description: Lens Epithelium-derived Growth Factor (LEDGF, also called as p75) has been shown to enhance survival of lens epithelial cells (LECs) against stress. LEDGF is a transcriptional activator. It protects the cells by binding to cis-stress response ((A/T) GGGG (T/A)), and heat shock (HSE; nGAAn) elements in the stress genes and activating their transcription. Originally, it was isolated as a co- activator required for transcriptional activation in human cell-free systems containing RNA Polymerase II and general initiation factors. LEDGF is expressed at all stages of development in a variety of organs and tissues. A second protein product, p52, can be produced from the same gene due to alternative splicing of pre-mRNA. In vitro, p52 was found to be more general and stronger transcriptional co-activator than LEDGF/p75. HIV-1 integrase (IN) forms a specific nuclear complex with p75 but not with p52, suggesting a role for p75's C-terminal region in retroviral integration. Recombinant p75 CTR is isolated from an E.coli strain that carries the C-terminal coding sequence of the human LEDGF/p75(amino acid 322-530) under the control of a T7 promoter. p75 has been applied in in vitro transcription assays, splicing assays, DNA and protein-protein interaction assays. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase, and RNase activity.
• Source: E.coli.
• Form: Liquid. Supplied in 20mM Tris-HCl pH7.9, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
• Activity: 20ng are sufficient for an in vitro transcription assay and 100ng are sufficient for a protein-protein interaction assay.
• Purity: >95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PSIP1 PC4 and SFRS1 interacting protein 1 [ Homo sapiens ]
• Synonyms: PSIP1; PC4 and SFRS1 interacting protein 1; p52; p75; PAIP; DFS70; LEDGF; PSIP2; MGC74712; transcriptional coactivator p52/p75; PC4 and SFRS1 interacting protein 2; OTTHUMP00000022753; OTTHUMP00000022754; OTTHUMP00000022756; CLL-associated antigen KW-7; Transcriptional coactivator p75/p52; Dense fine speckles 70 kDa protein; Lens epithelium-derived growth factor
• Gene ID: 11168
• mRNA Refseq: NM_001128217
• Protein Refseq: NP_001121689
• MIM: 603620
• UniProt ID: O75475
• Chromosome Location: 9p22.2
• Pathway: HIV Infection
• Function: DNA binding