Cat. No. : |
Acvrl1-498M |
Product overview : |
Recombinant Mouse Acvrl1 encoding the signal peptide from human CD33 joined with amino acid residues 23 - 119 of the extracellular domain of mouse Activin Receptor-Like Kinase 1was fused to the Fc region of human IgG1 via a polypeptide linker and expressed inNSO cells. |
Description : |
Activin A receptor type II-like 1, also known as ACVRL1, is a human gene. ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as "activin receptor-like kinase 1", or "ALK1". This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions. |
Molecular Weight : |
The predicted molecular weight of Recombinant Mouse ALK-1 is Mr 37.7 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 50-55 kDa. |
Source : |
Mammalian cells |
State Of Matter : |
Lyophilized. |
Purity : |
>95% by SDS Page and analyzed by silver stain. |
Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. |
Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
Biological Activity : |
Measured by its ability to inhibit recombinant Human BMP-9 induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 20 - 80 ng/ml in the presence of 2 ng/ml of recombinant Human BMP-9. |
Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |