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Recombinant Human ACACA, His-tagged

Cat. No.: ACACA-1848H
Product Overview: Recombinant Human ACC1 (amino acids 39 – end) with C-terminal His tag expressed inBaculovirusinfected Sf9 cellexpression system. MW= 292.5 kDa.
Description: Acetyl-CoA carboxylase 1 also known as ACC-alpha or ACCa is an enzyme that in humans is encoded by the ACACA gene. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA.
Source: Baculovirus infected Sf9 cell.
Purity: >60%.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity: 0.33 pmol/min/μg Reaction buffer: 4 μM ATP, 50mM HEPES (pH 7.5), 2mM MgCl2, 2mM potassium citrate. Substrate= 20 μM acetyl-CoA and 12mM NaHCO3. Incubate 1h 30min at RT, add 50μl Kinase-Glo® Plus (Promega) to 50 μl of reaction and read luminescence.
Formulated In: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 3 mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 50% glycerol and 3mM DTT.
Stability: >12 months at -80°C.
Gene Name: ACACA acetyl-Coenzyme A carboxylase alpha [ Homo sapiens ]
Synonyms: acetyl-Coenzyme A carboxylase alpha; ACC; ACAC; ACC1; ACCA; ACACA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha;EC 6.4.1.2
Gene ID: 31
mRNA Refseq: NM_198834.1
Protein Refseq: NP_942131.1
MIM: 200350
UniProt ID: Q13085
Chromosome Location: 17q21
Pathway: Fatty acid biosynthesis; Insulin signaling pathway; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism
Function: ATP binding; acetyl-CoA carboxylase activity; biotin binding; biotin carboxylase activity; ligase activity; metal ion binding; nucleotide binding; protein binding

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