||Recombinant human EPHB4 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=50kDa.
||EPHB4 is a member of the Eph family of receptor tyrosine kinases which play a role in regulating cell adhesion and cell movement during embryonic development. Furthermore, because tissue disorganization and abnormal cell adhesion, movement, and survival characterize the more advanced stages of cancer, the inappropriate functioning of Eph receptor could play a causal role in malignancy. EPHB4 is uniquely expressed in the vascular endothelial and endocardial cells and animal knockout studies with EPHB4 reveal a phenotype that is similar to Ephrin B2 gene knockdown. Furthermore, EPHB4 has been shown to be the major essential interaction partner of Ephrin B2 in angiogenesis.
||Sf9 insect cells.
||125 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 0.1 mM ATP, 30 μg/ml Poly (Glu:Tyr)4:1 substrate, and 2 μg /ml recombinant EPHB4.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 50% glycerol and 3mM DTT.
||>12 months at –80°C.