||Recombinant human NUTF2, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW =16.6 kDa(147aa).
||NUTF2 is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62 and Ran. This protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It binds directly to RanGDP and may modulate the steady-state distribution of Ran.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG
||> 95% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.