"NAPA" Related Products

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Recombinant Human NAPA, His-tagged

Cat. No.: NAPA-2815H
Product Overview: Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW =35.3 kDa(315aa), confirmed by MALDI-TOF.
Description: NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues.
Source: E.coli.
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Purity: > 95% by SDS - PAGE.
Form: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol.
Concentration: 1 mg/ml (determined by Bradford assay).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: NAPA N-ethylmaleimide-sensitive factor attachment protein, alpha [ Homo sapiens ]
Synonyms: NAPA; N-ethylmaleimide-sensitive factor attachment protein, alpha; SNAPA; alpha-soluble NSF attachment protein; SNAP-alpha; alpha-SNAP; alpha SNAP; N-ethylmaleimide-sensitive factor attachment protein alpha
Gene ID: 8775
mRNA Refseq: NM_003827
Protein Refseq: NP_003818
MIM: 603215
UniProt ID: P54920
Chromosome Location: 19q13.33
Pathway: Membrane Trafficking
Function: protein binding; syntaxin binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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