Active Recombinant Human Vitamin D (1,25- dihydroxyvitamin D3) Receptor

• Cat.No.: VDR-2516H
• Product Overview: Recombinant human VDR was expressed inbaculovirussystem and purified by an affinity column in combination with FPLC chromatography. 48 kDa.

Specification

• Cat. No.: VDR-2516H
• Description: The vitamin D endocrine system is critical for the proper development and maintenance of mineral ion homeostasis and skeletal integrity. Beyond these classical roles, recent evidence suggests that the bioactive metabolite of vitamin D, 1,25-dihydroxyvitamin D3, functions in diverse physiological processes, such as hair follicle cycling, blood pressure regulation, and mammary gland development. The biological effects of 1,25-(OH)2D3 are mediated through the vitamin D receptor (VDR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. The cellular effects of VDR signaling include growth arrest, differentiation and/or induction of apoptosis. VDR heterodimerizes with RXR and the liganded VDR-RXR heterodimer binds with high affinity to vitamin D response elements (VDREs) in the promoters of target genes. In addition, several nuclear receptor coactivators (SRC-1, DRIP) have been shown to interact with VDR and potentiate its transcriptional activity. In addition to treating disorders of mineral metabolism and diseases of the skeleton, such as rickets, osteoporosis, and renal osteodystrophy, VDR and 1,25-(OH)2D3 have significant therapeutic potential for pathologies such as cancer, autoimmune syndromes, and psoriasis.
• Source: baculovirus.
• Applications: Recombinant VDR can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro acetylation assay; and 4) for cell growth assay.
• Transcript Variant: This variant lacks an alternate exon in the 5" UTR compared to variant 2. Variants 1 and 2 encode the same protein.
• Activity: 1 unit equals 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20µl reaction; 50-100 units are sufficient for reconstituted transcription assay and 100-200 units are sufficient for a protein-protein interaction assay or an acetylation assay.
• Quality Control: The purified protein is greater than 95% homogeneous based on SDS-PAGE gel analysis and contains no detectable proteases, DNase or RNase activity.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Storage Conditions: Store at -80°C.
• Pathways: Gene Expression

Gene Information

• Gene Name: VDR vitamin D (1,25-dihydroxyvitamin D3) receptor [ Homo sapiens ]
• Synonyms: VDR; vitamin D (1,25-dihydroxyvitamin D3) receptor; NR1I1; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
• Gene ID: 7421
• mRNA Refseq: NM_000376
• Protein Refseq: NP_000367
• MIM: 601769
• UniProt ID: P11473
• Chromosome Location: 12q12-q14
• Function: metal ion binding; protein binding; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; vitamin D response element binding; vitamin D3 receptor activity; zinc ion binding